(A–B) Representative Western blot (A) and quantification (B) of OPTN, p62, TAX1BP1, and NDP52 in total brain, cortex, and hippocampus. Mean ± SEM; n = 3 biological replicates. (C–E) Representative image of endogenous OPTN in the soma (C), distal axon (D), and dendrite (E). (F–H) Representative image of endogenous p62 in the soma (F), distal axon (G), and dendrite (H). Red arrows in panels C and F depict the axon labeled by anti-Neurofilament H (NF-H) and anti-Tau, respectively; an enlarged image of the soma is shown in the inset. Corresponding line scans for the axon and dendrite are shown to the right of the representative images; red arrows indicate OPTN and p62 puncta. Scale bars: soma, 20 μm; axons and dendrites, 5 μm.