(A) Expression pattern of the BLsensR57C10 line. Gal4 is highly expressed in the brain lobes and SOG until the anterior boundary of SCR expression. (B) Schematic of the experimental design. Each one of the genotypes was subjected to the treatment protocol, which induces the expression of proapoptotic genes (rpr and hid) in mid embryonic stages resulting in apoptosis when the larvae hatch. Higher levels of apoptosis were induced by increasing temperature to 33°C 24 hr after hatching. Control animals were kept under non-activating (22°C) conditions. The behavioural experiments were conducted at day 3. (C) Quantification of active larvae in the treatment conditions. w1118 was used as a control. Asterisk denotes that at day 4 a few larvae have moulted. (D) Apoptosis and larval size. The expression of Death Caspase-1 (anti-CPD-1 in yellow) was used as a readout of cell death. Nervous systems were evaluated at hatching (i, vi), at day 1 (ii, vii) and at day 2 (iii) and day 3 (iv,viii). Neuronal death is observed in the brain lobes of BLsensR57C10 > rpr,hid larvae and in the entire nervous system of R57C10 > rpr,hid animals at hatching and day 1. At day 2, R57C10 > rpr,hid were all dead indicating that a high level of apoptosis was reached. The number of neurons ongoing apoptosis started declining in BLsensR57C10 > rpr,hid larvae brains until day three when CPD-1 was only observed in granules. Larvae with brain lobes death (v) are smaller than control animals (viii) likely due to decrease food intake. (E) Scatter plot and average exponents (± SD) of the truncated Levy power-law best model fits to exploratory pattern. R57C10 > rpr,hid have a mean exponent (µ) of 2.1 close to the theoretically near-optimum search pattern (µoptopt2). The data distribution was not normal using D'Agostino-Pearson normality test. A Mann Whitney test showed significant differences between the BLsensR57C10 > rpr,hid control animals raised at 22°C and the treatment with induced apoptosis. U = 222.5, ncontrol = 62, ntreatment = 23, p<0.0001.