(A) Experimental design of the restraint stress protocol. (B) Summary of nine cages representing the SCTT ranks and winning times as a function of SCTT trials over the 8 days of test. (C) Time spent in tube as a function of rank pairing (F5,35=18.19, p<0.0001, one-way ANOVA; *p<0.05, ***p<0.001, Bonferroni’s test, n = 7 per rank pairing). (D) Anxiety-like behaviors measured as the percent time spent in the open arms of an elevated plus maze after segregation into high rank vs low rank mice (p*<0.05, unpaired t-test, two-tailed, n = 14 per group). (E) Anxiety-related behaviors measured in the open-field, including latency to first enter the center of the arena (*p<0.05, unpaired t-test, two-tailed n = 14 per group) and time in center zone (n.s. unpaired t-test, two-tailed n = 14 per group). Locomotor activity is measured as the distance travelled in the OF (n.s. unpaired t-test, two-tailed n = 14 per group). (F) Social interaction (SI) test measured after chronic restraint stress protocol in high rank vs low rank animals (Social avoidance score: Interaction: F1,21=7.75, p<0.05; rank effect: F1,21=1.18, p>0.05; stress effect: F1,21=7.15, p<0.05, two-way ANOVA; p*<0.05, **p<0.01, Bonferroni’s test, n = 6–7 per group/Time in interaction zone: Interaction: F1,21=12.80, p<0.005; rank effect: F1,21=0.85, p<0.05; stress effect: F1,21=12.21, p<0.005, two-way ANOVA; p*<0.05, **p<0.005, Bonferroni’s test, n = 6–7 per group/Time in corner zone: Interaction: F1,21=3.29, p>0.05; rank effect: F1,21=0.28, p<0.05; stress effect: F1,21=2.14, p>0.05, two-way ANOVA, n = 6–7 per group). Data are displayed as mean ± SEM.