Unadhered lipoplex containing four dsDNAs sits above the endosomal bilayer (I). After initial stalk formation (II), a wide hemifusion diaphragm is formed (III). A pore is formed in one of the …
First the AA geometry and force field of the dsDNA was translated into their CG counterpart (A). Then the dsDNA was stacked on top of a bilayer composed of DOTAP and DOPE in a 1:4 ratio …
The radius of gyration for the solvated small lipoplex over time. Over the last 2 µs of the simulation the radius of gyration increases less than a quarter of an Ångstrom. We therefore concluded …
Upon solvating the periodic lipoplex an extra layer of coating lipids was added. We used the approximate surface area of the lipoplex and divided it by the APL of the DOTAP:DOPE mixture (1:4; 0.63 …
The membrane compositions for the fusion experiments, and the lipoplex formulations are indicated in molar ratios. All PC/PS heads were combined with all tails, resulting in six different lipids per …
Snapshots of endosome bilayers after 1 µs of equilibration after being generated by insane. From left to right we show the most, more unsaturated and short lipids bilayer respectively (see Figure 2).…
The number of successful transfections and amount of transfected DNA considering different lipid compositions. Each composition had 5 repeats with four dsDNA fragments each. Therefore the maximum …
Shown are the observables for the lipoplex-membrane fusion experiments. Each experiment was performed five times (one repeat per column). For each simulation the first 10 µs were taken into account. …
Shown are the observables for the lipoplex-membrane fusion experiments. Each experiment was performed five times (one repeat per column). For each simulation the first 10 µs were taken into account. …
Shown are the observables for the lipoplex-membrane fusion experiments. Each experiment was performed five times (one repeat per column). For each simulation the first 10 µs were taken into account. …
Shown are the observables for the lipoplex-membrane fusion experiments. Each experiment was performed five times (one repeat per column). For each simulation the first 10 µs were taken into account. …
Shown are the observables for the lipoplex-membrane fusion experiments. Each experiment was performed five times (one repeat per column). For each simulation the first 10 µs were taken into account. …
The large lipoplex (A) showed the same stable HII structure as the small lipoplex with dsDNA inside the aqueous channels. Small additional connecting channels are also present, as indicated. …
The solvated large lipoplex contained channels: regular dsDNA/aqueous channels are indicated in peach (A); an open channel is depicted with a magenta circle (B); and aqueous connecting channels are …
Fusion of a small lipoplex containing 4 fragments of dsDNA. The headgroups of the lipoplex lipids (DOTAP:1, DOPE:4) are depicted in blue and the bilayer lipids (short lipids Figure 2) are depicted …
Fusion of the large lipoplex from inside the endosomal vesicle. The headgroups of the endosomal and lipoplex lipids are subsequently blue and orange. The endosomal lipids have dark grey tails and …
Components of the lipoplex closer than 4 nm to the endosome are colored blue, the endosomal lipids are grey and the dsDNA inside the lipoplex is yellow/pink. Within 1 µs the lipoplex reorients …
DOTAP_validation.
lipoplex_lipid-properties.