Inter-and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex
- University of Edinburgh, United Kingdom
Abstract
Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.
Data availability
Processed data used for analyses and all associated code is available from the GitHub page for the project (https://github.com/MattNolanLab/Inter_Intra_Variation).Raw data has been made available from our institutional repository and can be found under the DOI 10.7488/ds/2765. Scripts that generate the processed data from the raw data will be made available from our GitHub site. We expect to complete documention of these scripts in the next few weeks. We will make the data and scripts freely available when this is complete.
Article and author information
Author details
Funding
Wellcome (200855/Z/16/Z)
- Matthew F Nolan
Biotechnology and Biological Sciences Research Council (BB/L010496/1)
- Matthew F Nolan
Biotechnology and Biological Sciences Research Council (BB/1022147/1)
- Matthew F Nolan
Biotechnology and Biological Sciences Research Council (BB/H020284/1)
- Matthew F Nolan
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All experimental procedures were performed under a United Kingdom Home Office license (PC198F2A0) and with approval of the University of Edinburgh's animal welfare committee.
Copyright
© 2020, Pastoll et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Inter-and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex
eLife 9:e52258.
https://doi.org/10.7554/eLife.52258
Further reading
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