(A) FACS profile of HG cells and qPCR validation of marker genes. Sca1 was used to exclude epidermis. CD34 was used to exclude HFSC and HG cells were gated as Sca1-, CD34-, Pcadhigh. (B) FACS profile of Matrix cells and qPCR validation of marker genes. Sca1 was used to exclude epidermis and cells were gated as Sca1-, Pcadhigh. (C) ELISA detection of RA in different cell populations in WT and Krt6-CreER::RBP-J HFs. (D) FACS profiles of McSCs from telogen HFs. After trypsin treatment to collect all cells in hair follicles from telogen dorsal skin, McSCs were gated as CD45-, integrin α6-, c-Kit+ population. CD45 was used to exclude immune cells, integrin α6- was used to exclude epithelial cells. (E) Cytospin staining images and quantification of melanocyte marker DCT in FACS isolated populations. Note close to 100% of sorted CD45-, integrin α6-, c-Kit+ cells are DCT+, but none of integrin α6+ epithelial cells are DCT+. (F) QPCR analysis of epithelial cell marker Krt14 and melanocyte marker DCT in FACS isolated cells. Data are expressed as mean ± SD ≥ 20 follicles are quantified each mouse. N = 3. (*) p<0.05.