Antagonistic control of C. elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2
- University of Montana, United States
Abstract
Stem cells support tissue maintenance, but the mechanisms that coordinate the rate of stem cell self-renewal with differentiation at a population level remain uncharacterized. We find that two PUF family RNA-binding proteins FBF-1 and FBF-2 have opposite effects on C. elegans germline stem cell dynamics: FBF-1 restricts the rate of meiotic entry, while FBF-2 promotes both cell division and meiotic entry rates. Antagonistic effects of FBFs are mediated by their distinct activities towards the shared set of target mRNAs, where FBF-1-mediated post-transcriptional control requires the activity of CCR4-NOT deadenylase, while FBF-2 is deadenylase-independent and might protect the targets from deadenylation. These regulatory differences depend on protein sequences outside of the conserved PUF family RNA-binding domain. We propose that the opposing FBF-1 and FBF-2 activities serve to modulate stem cell division rate simultaneously with the rate of meiotic entry.
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All data generated or analysed during this study are included in the manuscript and supporting files.
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Author details
Ekaterina Voronina
Funding
National Institute of General Medical Sciences (GM109053)
- Ekaterina Voronina
National Institute of General Medical Sciences (P20GM103546)
- Ekaterina Voronina
American Heart Association (18PRE34070028)
- Xiaobo Wang
Montana Academy of Sciences
- Xiaobo Wang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Antagonistic control of C. elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2
eLife 9:e52788.
https://doi.org/10.7554/eLife.52788
