(A) Microscopic image of platelets prepared by pipetting. Only a small number of platelet aggregates can be identified, while most of the cells in the image are leukocytes and single platelets. (B) Microscopic image of platelets prepared by vortexing. Platelet-platelet aggregates and platelet-leukocyte aggregates can be identified in the image, while only a small population of the platelets remained as single platelets. (C) Microscopic image of platelets without fixation at 0 hr. Platelet-platelet aggregates, platelet-leukocyte aggregates, and single platelets can be identified in the image. (D) Microscopic image of platelets without fixation at 3 hr. Most of the platelet aggregates dissociated into single platelets and leukocytes.