A moth odorant receptor highly expressed in the ovipositor is involved in detecting host-plant volatiles
- State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, China
- CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, China
- Forestry College, Henan University of Science and Technology, China
Figures
Figure 1 with 1 supplement
Gene expression of HassOR31 in different tissues of H. assulta.
(A) Tissue expression profiles of putative chemosensory receptor genes identified in the pheromone gland-ovipositor complex (PG-OV) of H. assulta. (B) TPM values of HassOR31 and HassORco in …
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Figure 1—source data 1
Source data for Figure 1A and C.
- https://cdn.elifesciences.org/articles/53706/elife-53706-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
Tissue expression profiles of some putative chemosensory related genes identified in the pheromone gland-ovipositor complex of H. assulta.
TPM values are listed below different tissues. OBP, odorant binding protein; CSP, chemosensory protein; GOBP, general odorant binding protein; PBP, pheromone binding protein; SNMP, sensory neuron …
Figure 2 with 1 supplement
Localization of HassOR31 and HassORco expression in cells of H. assulta ovipositors.
Double-FISH with female ovipositors using combinations of labeled OR probes and visualization of cells bearing distinct HassOR31 transcripts by red (DIG) (A1, B1, C1) and HassORco transcripts by …
Figure 2—figure supplement 1
More examples of two-color in situ hybridization visualizing the combinations of HassOR31 (red) and HassORco (green) in the ovipositor of H. assulta.
Arrows indicate the cell location. Scale bars: 10 μm.
Figure 3
Functional analyses of chemosensory receptors in Xenopus oocytes.
(A) Inward current responses of Xenopus oocytes with expressed HassOR31/HassORco, HassOR31 alone, or HassOR31/HassiGluR7 in response to odorants (10−4 M solution). Odorants were applied for 8 s at …
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Figure 3—source data 1
Source data for Figure 3B and C.
- https://cdn.elifesciences.org/articles/53706/elife-53706-fig3-data1-v2.xlsx
Figure 4
Scanning electron micrographs (SEM) of the ovipositor of H. assulta and its associated sensilla.
(A) An overview of the extended tip of the ovipositor of H. assulta, which contains two anal papillae surrounding the ovipore. (B) Details of the papilla close to the ovipore. There are four types …
Figure 5
Single sensillum recordings (SSR) of putative chemosensory sensilla in an H. assulta ovipositor.
(A) The firing rate of a putative chemosensory sensilla to several plant volatile and sex pheromone components. Chemicals with * induced significantly electrophysiological responses at p<0.05 …
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Figure 5—source data 1
Source data for Figure 5A and C.
- https://cdn.elifesciences.org/articles/53706/elife-53706-fig5-data1-v2.xlsx
Figure 6 with 1 supplement
Oviposition preference of the antennae amputated and intact mated females of H. assulta.
(A) Number of eggs on the hot pepper treated gauze and control gauze. Two and three asterisks indicate significant difference (p<0.01 and p<0.001, paired t test). (B) Oviposition preference index …
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Figure 6—source data 1
Source data for Figure 6A, B, C and D.
- https://cdn.elifesciences.org/articles/53706/elife-53706-fig6-data1-v2.xlsx
Figure 6—figure supplement 1
The set-up of oviposition choice tests and the spread of eggs laid by mated females of H. assulta.
(A) The choice test 1, the pepper treated gauzes vs control gauzes. The gauze was equally divided into four sections. The diameter of the hot pepper fruit discs is 1.5 cm. To avoid the moths …
Videos
Video 1
Z-stack video of two-color in situ hybridization visualizing the combinations of HassOR31 (red) and HassORco (green) in the ovipositor of H. assulta.
White arrows indicate the cells only expressing HassOR31, and yellow arrow the cell co-expressing HassOR31 and HassORco.
Video 2
An exemplary SSR experiment video.
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Helicoverpa assulta) | 18S ribosomal RNA gene | NCBI | GenBank: EU057177.1 | |
| Commercial assay, kit | RNeasy Plus Universal Mini Kit | Qiagen | Cat# 73404 | |
| Commercial assay, kit | Dynabeads mRNA purification kit | Invitrogen | Cat# 61006 | |
| Commercial assay, kit | Q5 High-Fidelity DNA Polymerase | NEB | Cat# M0491 | |
| Commercial assay, kit | M-MLV reverse transcriptase | Promega | Cat# M1701 | |
| Commercial assay, kit | SYBR Premix Ex TaqII | Takara | Cat# RR820 | |
| Commercial assay, kit | T7/SP6 RNA transcription system | Roche | Cat# 10999644001 | |
| Commercial assay, kit | mMESSAGE mMACHINE SP6 | Ambion | Cat# AM1340 | |
| Software, algorithm | Trimmomatic | Trimmomatic | RRID:SCR_011848 | |
| Software, algorithm | FastQC | FastQC | RRID:SCR_014583 | |
| Software, algorithm | Trinity | Trinity | RRID:SCR_013048 | |
| Software, algorithm | RSEM | RSEM | RRID:SCR_013027 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism | RRID:SCR_002798 | 7.0 |
| Software, algorithm | ZEN Digital Imaging for Light Microscopy | ZEN Digital Imaging for Light Microscopy | RRID:SCR_013672 | 2012 |
| Software, algorithm | Adobe Illustrator | Adobe systems | RRID:SCR_014198 | CS6 |
| Software, algorithm | pCLAMP software | pCLAMP software | RRID:SCR_011323 |
Additional files
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Supplementary file 1
Expression values of putative chemosensory receptors in the pheromone gland-ovipositor complex of H. assulta (Hass) and H. armigera (Harm).
OR, odorant receptor; GR, gustatory receptor; IR, antennal ionotropic receptor; iGluR, ionotropic glutamate receptor.
- https://cdn.elifesciences.org/articles/53706/elife-53706-supp1-v2.docx
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Supplementary file 2
Primers used for qRT-PCR, RT-PCR, probe synthesis and full-length cDNA cloning.
- https://cdn.elifesciences.org/articles/53706/elife-53706-supp2-v2.docx
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Supplementary file 3
Tested compounds for functional analysis of HassOR31.
- https://cdn.elifesciences.org/articles/53706/elife-53706-supp3-v2.docx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/53706/elife-53706-transrepform-v2.docx
