Strain, strain background (Mus musculus) | B6.129S4(CG)-Arntltm1Weit/J | The Jackson Laboratory | JAX: 007668 | Carry loxp sites flanking exon 8 of the Bmal1 gene |
Strain, strain background (Mus musculus) | B6.129-Hif1atm3Rsjo/J | The Jackson Laboratory | JAX: 007561 | Carry loxp sites flanking exon 2 of the Hif1a gene |
Strain, strain background (Mus musculus) | B6.129P2-Lyz2tm1(cre)lfo/J | The Jackson Laboratory | JAX: 004781 | Myeloid-specific Cre recombinase expression |
Antibody | Mouse monoclonal anti-Bmal1 (clone B-1) | Santa Cruz Biotechnology | Cat# sc365645; RRID:AB_10841724 | WB (1:1000) |
Antibody | Rabbit polyclonal anti-Hif-1α | Novus Biologicals | Cat# NB100-449; RRID:AB_10001045 | WB (1:1000) |
Antibody | Rabbit monoclonal anti-Bnip3 (clone EPR4034) | Abcam | Cat# ab109362; RRID:AB_10864714 | WB (1:1000) |
Antibody | Rabbit polyclonal anti-β-tubulin | Cell Signaling Technology | Cat# 2146; RRID:AB_2210545 | WB (1:1000) |
Antibody | Rabbit monoclonal anti-β-Actin (clone 13E5) | Cell Signaling Technology | Cat# 4970; RRID:AB_2223172 | WB (1:1000) |
Antibody | Rat monoclonal anti-CD45 (clone 30-F11), PerCP/Cy5.5-conjugated | Biolegend | Cat# 103132; RRID:AB_893340 | Flow cytometry (1 μL per test) |
Antibody | Armenian hamster monoclonal anti-CD3ε (clone 145–2 C11), PE/Cy7-conjugated | Biolegend | Cat# 100320; RRID:AB_312685 | Flow cytometry (2.5 μL per test) |
Antibody | Rat monoclonal anti-CD8a (clone 53–6.7), Alexa Fluor 700-conjugated | Biolegend | Cat# 100730; RRID:AB_493703 | Flow cytometry (0.5 μL per test) |
Antibody | Mouse monoclonal anti-NK1.1 (clone PK136), APC-conjugated | Biolegend | Cat# 108710 | Flow cytometry (5 μL per test) |
Antibody | Rat monoclonal anti-F4/80 (clone BM8), Alexa Fluor 488-conjugated | Biolegend | Cat# 123120; RRID:AB_893479 | Flow cytometry (5 μL per test) |
Antibody | Rat monoclonal anti-F4/80 (clone BM8), APC-conjugated | Biolegend | Cat# 123115; RRID:AB_893493 | Flow cytometry (2.5 μL per test) |
Antibody | Rat monoclonal anti-Ifn-γ (clone XMG1.2), PE-conjugated | ThermoFisher Scientific | Cat# 12-7311-81, RRID:AB_466192 | Flow cytometry (1 μL per test) |
Peptide, recombinant protein | Recombinant murine interferon-γ | Peprotech | Cat# 315–05 | Used 10 ng/mL final concentration |
Peptide, recombinant protein | Recombinant murine interleukin-4 | Peprotech | Cat# 214–14 | Used 10 ng/mL final concentration |
Sequence-based reagent | 36b4_F | This paper | qPCR primer | AGATGCAGCAGATCCGCAT |
Sequence-based reagent | 36b4_R | This paper | qPCR primer | GTTCTTGCCCATCAGCACC |
Sequence-based reagent | Arg1_F | This paper | qPCR primer | CGTAGACCCTGGGGAACACTAT |
Sequence-based reagent | Arg1_R | This paper | qPCR primer | TCCATCACCTTGCCAATCCC |
Sequence-based reagent | Bckdhb_F | This paper | qPCR primer | TGGGGCTCTCTACCATTCTCA |
Sequence-based reagent | Bckdhb_R | This paper | qPCR primer | GGGGTATTACCACCTTGATCCC |
Sequence-based reagent | Bmal1_F | This paper | qPCR primer | AGGATCAAGAATGCAAGGGAGG |
Sequence-based reagent | Bmal1_R | This paper | qPCR primer | TGAAACTGTTCATTTTGTCCCGA |
Sequence-based reagent | cMyc_F | This paper | qPCR primer | CAGCGACTCTGAAGAAGAGCA |
Sequence-based reagent | cMyc_R | This paper | qPCR primer | GACCTCTTGGCAGGGGTTTG |
Sequence-based reagent | Cry1_F | This paper | qPCR primer | CACTGGTTCCGAAAGGGACTC |
Sequence-based reagent | Cry1_R | This paper | qPCR primer | CTGAAGCAAAAATCGCCACCT |
Sequence-based reagent | Gclc_F | This paper | qPCR primer | CATCCTCCAGTTCCTGCACA |
Sequence-based reagent | Gclc_R | This paper | qPCR primer | ATGTACTCCACCTCGTCACC |
Sequence-based reagent | Hif1a_F | This paper | qPCR primer | GAACGAGAAGAAAAATAGGATGAGT |
Sequence-based reagent | Hif1a_R | This paper | qPCR primer | ACTCTTTGCTTCGCCGAGAT |
Sequence-based reagent | Hmox1_F | This paper | qPCR primer | CAGAGCCGTCTCGAGCATAG |
Sequence-based reagent | Hmox1_R | This paper | qPCR primer | CAAATCCTGGGGCATGCTGT |
Sequence-based reagent | Il1b_F | This paper | qPCR primer | AGCTTCAGGCAGGCAGTATC |
Sequence-based reagent | Il1b_R | This paper | qPCR primer | AAGGTCCACGGGAAAGACAC |
Sequence-based reagent | Ldha_F | This paper | qPCR primer | GCGTCTCCCTGAAGTCTCTT |
Sequence-based reagent | Ldha_R | This paper | qPCR primer | GCCCAGGATGTGTAACCTTT |
Sequence-based reagent | Mgl2_F | This paper | qPCR primer | ccttgcgtttgtcaaaacatgac |
Sequence-based reagent | Mgl2_R | This paper | qPCR primer | ctgaggcttatggaactgaggc |
Sequence-based reagent | Mmsdh_F | This paper | qPCR primer | GAGGCCTTCAGGTGGTTGAG |
Sequence-based reagent | Mmsdh_R | This paper | qPCR primer | GATAGATGGCATGGTCTCTCCC |
Sequence-based reagent | Nfe2l2_F | This paper | qPCR primer | GGTTGCCCACATTCCCAAAC |
Sequence-based reagent | Nfe2l2_R | This paper | qPCR primer | GCAAGCGACTCATGGTCATC |
Sequence-based reagent | Nfkb1_F | This paper | qPCR primer | CCTGCTTCTGGAGGGTGATG |
Sequence-based reagent | Nfkb1_R | This paper | qPCR primer | GCCGCTATATGCAGAGGTGT |
Sequence-based reagent | Nqo1_F | This paper | qPCR primer | TCTCTGGCCGATTCAGAGTG |
Sequence-based reagent | Nqo1_R | This paper | qPCR primer | TGCTGTAAACCAGTTGAGGTTC |
Sequence-based reagent | Nr1d2_F | This paper | qPCR primer | TCATGAGGATGAACAGGAACCG |
Sequence-based reagent | Nr1d2_R | This paper | qPCR primer | CGGCCAAATCGAACAGCATC |
Sequence-based reagent | Ppard_F | This paper | qPCR primer | CAGCCTCAACATGGAATGTC |
Sequence-based reagent | Ppard_R | This paper | qPCR primer | TCCGATCGCACTTCTCATAC |
Sequence-based reagent | Pparg_F | This paper | qPCR primer | CAGGAGCCTGTGAGACCAAC |
Sequence-based reagent | Pparg_R | This paper | qPCR primer | ACCGCTTCTTTCAAATCTTGTCTG |
Sequence-based reagent | Slc7a2_F | This paper | qPCR primer | CCCGGGATGGCTTACTGTTT |
Sequence-based reagent | Slc7a2_R | This paper | qPCR primer | AGGCCATCACAGCAGAAATGA |
Sequence-based reagent | Slc7a8_F | This paper | qPCR primer | GAACCACCCGGGTTCTGAC |
Sequence-based reagent | Slc7a8_R | This paper | qPCR primer | TGATGTTCCCTACAATGATACCACA |
Sequence-based reagent | Slc7a11_F | This paper | qPCR primer | ATCTCCCCCAAGGGCATACT |
Sequence-based reagent | Slc7a11_R | This paper | qPCR primer | GCATAGGACAGGGCTCCAAA |
Sequence-based reagent | Stat3_F | This paper | qPCR primer | TGGCAGTTCTCGTCCAC |
Sequence-based reagent | Stat3_R | This paper | qPCR primer | CCAGCCATGTTTTCTTTGC |
Sequence-based reagent | Bmal1_F | This paper | Cloning primers | GGCGAATTCGCGGACCAGAGAATGGAC |
Sequence-based reagent | Bmal1_R | This paper | Cloning primers | GGGCTCGAGCTACAGCGGCCATGGCAA |
Cell line (Mus musculus) | RAW264.7 macrophages | ATCC | qPCR primer | TIB-71 |
Cell line (Mus musculus) | B16-F10 melanoma cells | ATCC | qPCR primer | CRL-6475 |
Recombinant DNA reagent | pBABE-puro retroviral expression vector (plasmid) | Addgene | Plasmid #1764 | |
Recombinant DNA reagent | pBABE-Bmal1 (plasmid) | This study | | For stable overexpression of mouse Bmal1 |
Chemical compound, drug | Lipopolysaccharides from Escherichia coli K-235 | Sigma-Aldrich | Cat# L2143 | Final concentration used varied as indicated |
Chemical compound, drug | Dimethyl malonate | Sigma-Aldrich | Cat# 136441; CAS: 108-59-8 | Used 10 mM final concentration |
Chemical compound, drug | Sodium succinate dibasic | Sigma-Aldrich | Cat# 14160; CAS: 150-90-3 | Used 10 mM final concentration |
Chemical compound, drug | N-acetyl-L-cysteine | Sigma-Aldrich | Cat# A9165; CAS: 616-91-1 | Final concentration used varied as indicated. |
Chemical compound, drug | 2-deoxy-D-glucose | Sigma-Aldrich | Cat# D8375; CAS: 154-17-6 | Used 50 mM final concentration |
Chemical compound, drug | Quant-iT ribogreen RNA reagent | ThermoFisher Scientific | Cat# R11491 | |
Chemical compound, drug | Mitotracker green FM | ThermoFisher Scientific | Cat# M7514 CAS: 201860-17-5 | Used 100 nM final concentration |
Chemical compound, drug | Mitosox red superoxide indicator | ThermoFisher Scientific | Cat# M36008 | Used 5 μM final concentration |
Chemical compound, drug | 2-NBDG | ThermoFisher Scientific | Cat# N13195 | Used 10 μM final concentration |
Chemical compound, drug | Fixable viability dye eFluor 455uv | ThermoFisher Scientific | Cat# 65-868-14 | Flow cytometry (0.5 μL per test) |
Chemical compound, drug | Fixable viability dye eFluor 506 | ThermoFisher Scientific | Cat# 65-0866-14 | Flow cytometry (0.5 μL per test) |
Chemical compound, drug | Oligomycin | Abcam | Cat# ab141829; CAS: 1404-19-9 | Used 2 μM final concentration |
Chemical compound, drug | Rotenone | Abcam | Cat# ab143145 CAS: 83-79-4 | Used 1 μM final concentration |
Chemical compound, drug | Antimycin A | Sigma-Aldrich | Cat# A8674; CAS: 1397-94-0 | Used 1 μM final concentration |
Chemical compound, drug | Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) | Santa Cruz Biotechnology | Cat# sc203578 CAS: 370-86-5 | Used 0.5 μM final concentration |
Chemical compound, drug | Phorbol 12-myristate 13-acetate (PMA) | Sigma-Aldrich | Cat# P8139; CAS: 16561-29-8 | Used 20 ng/mL final concentration |
Chemical compound, drug | Ionomycin calcium salt from Streptomyces conglobatus | Sigma-Aldrich | Cat# I0634; CAS: 56092-82-1 | Used 1 μg/mL final concentration |
Chemical compound, drug | Brefeldin A | Cell Signaling Technology | Cat# 9972S; CAS: 20350-15-6 | Used 10 μg/mL final concentration |
Chemical compound, drug | BCA protein assay kit | ThermoFisher Scientific | Cat# 23227 | |
Chemical compound, drug | Seahorse XF24 FluxPak | Agilent | Cat# 102070–00 | |
Chemical compound, drug | Foxp3/Transcription factor staining buffer set | ThermoFisher Scientific | Cat# 00-5523-00 | |
Chemical compound, drug | TruSeq stranded mRNA library prep kit | Illumina | Cat# RS-122–2101 | |
Chemical compound, drug | Dynabeads sheep anti-rat IgG | ThermoFisher Scientific | Cat # 11035 | |
Software, algorithm | STRING | von Mering, 2004 | https://string-db.org/ | |
Software, algorithm | DAVID | Huang et al., 2009 | https://david-d.ncifcrf.gov/ | |
Other | Thioglycollate medium | Sigma-Aldrich | Cat# T9032 | |
Other | Bovine serum albumin, fatty acid free | Gemini Bio-Products | Cat# 700–107P | |
Other | Collagen I protein, rat tail | ThermoFisher Scientific | Cat# A1048301 | |
Other | Collagenase type IV | ThermoFisher Scientific | Cat# 17104019 | |
Other | Recombinant DNase I, RNase-free | Affymetrix | Cat# 78411 | |