(A) Generation of ΔDsk (A1) and ΔDskGAL4 (A2). Dashed boxes indicate the region replaced by 3P3-RFP (A1) or GAL4 (A2) cassette. (B) Male adult brains of the indicated genotypes were stained with …
(A) Quantification of Dsk mRNA by qRT-PCR in the homozygotes, heterozygotes, and wildtypes. The Dsk transcripts were not detectable in ΔDsk /ΔDsk and ΔDskGAL4/ΔDskGAL4 mutants. Triplicate assays per …
(A) ΔDsk mutants show reduced number of wing threats compared to wildtype and heterozygous controls. ***p<0.001 (Kruskal-Wallis and post-hoc Mann-Whitney U tests).
(A–B) RU486 induced overexpression of Dsk increased number of lunges per minute after initiation (A) and shortened fighting latency (B) in males. **p<0.01, ***p<0.001, n.s. indicates no significant …
(A) Head butts frequency of female-female aggression. Homozygotes of ΔDsk showed a significant decrease in head butts compared to heterozygotes or wildtypes. (B) Fighting latency of female-female …
(A) Courtship indices for male-male courtship. (B) Courtship indices for male-female courtship. (C) The locomotive speed of ΔDsk mutants. (D) The feeding behavior of ΔDsk mutants. ΔDsk mutants show …
The aggression chamber consists of four acrylic plates. The bottom plate (Plate 4) contains 12 wells for food patches (diameter: 8 mm; depth: 3 mm). The lower (Plate 3) and upper plates (Plate 2) …
(A) Generation of DskGAL4 Knock-in line. GAL4 was fused to the end of open reading frame (ORF) of Dsk with a T2A peptide linker. (B–C) Anatomical features of DSK neurons revealed by DskGAL4 driven UA…
(A–D) Characterization of individual DskGAL4 neurons using MultiColor FlpOut (MCFO) method in females (A). Eight DSK-expressing neurons were classified into three neuronal cell types (B–D). Scale …
(A) The locomotive speed of TNT experiment. (B) The locomotive speed of trpA1 experiment. Inactivation or activation of DskGAL4 neurons could not affect locomotion behavior. ns. indicates no …
(A) A negative correlation between the number of type I plus type II neurons inhibited and the lunge numbers of individual flies. (B) A negative correlation between the number of type I neurons …
The aggression chamber consists of four acrylic plates. The bottom plate (Plate 4) contains 12 wells for food patches (diameter: 8 mm; depth: 3 mm). The lower (Plate 3) and upper plates (Plate 2) …
(A) Schematic view of CCKLR-17D1 gene locus and generation of ΔCCKLR-17D1 and CCKLR-17D1GAL4 Knock-in lines. The last two exons of CCKLR-17D1 gene were replaced by 3P3-RFP to generate ΔCCKLR-17D1. …
(A) Quantification of CCKLR-17D1 and CCKLR-17D3 mRNA by qRT-PCR in the CCKLR mutants and wildtypes. CCKLR-17D1 and CCKLR-17D3 transcripts were not detected in ΔCCKLR-17D1/y and ΔCCKLR-17D3/y …
(A) Generation of CCKLR-17D3GAL4 Knock-in line. GAL4 was fused to the end of open reading frame (ORF) of CCKLR-17D3 with a T2A peptide linker. (B–C) Projection patterns of CCKLR-17D3GAL4 neurons in …
(A) The locomotive speed of ΔCCKLR-17D1 and ΔCCKLR-17D3 mutants. ns. indicates no significant difference (Kruskal-Wallis and post-hoc Mann-Whitney U tests).
(A–B) RNAi knock-down of CCKLR-17D1 decreases inter-male aggression. (A) Number of lunges per minute after initiation. (B) Fighting latency. elav-GAL4; UAS-dcr2 or CCKLR-17D1GAL4 drives CCKLR-17D1 …
The aggression chamber consists of four acrylic plates. The bottom plate (Plate 4) contains 12 wells for food patches (diameter: 8 mm; depth: 3 mm). The lower (Plate 3) and upper plates (Plate 2) …
(A) Transsynaptic labeling by trans-Tango method identifies DSK neurons as postsynaptic partners of a subset of P1 neurons. Expression of the trans-Tango ligand in a subset of P1 neurons (red) (A1) …
(A) Negative control demonstrates lack of postsynaptic signals (anti-HA, magenta) (A2) for the trans-Tango experiment. Red, anti-DSK antibody staining (A1). (B–C) Negative controls for the GRASP …
(A–B) Raster plots of unilateral wing extensions during 10 min. (C–D) The number (C) and total duration (D) of unilateral wing extensions in (A–B) during thermogenetic activation of a subset of P1 …
The aggression chamber consists of four acrylic plates. The bottom plate (Plate 4) contains 12 wells for food patches (diameter: 8 mm; depth: 3 mm). The lower (Plate 3) and upper plates (Plate 2) …
(A) Illustration of patch-clamp recording on DSK neurons (magenta). ATP-gated ion channel P2X2 was expressed in R71G01-LexA labeled P1 neurons for chemogenetic activation of P1 neurons. Genotype: +/y…
(A–B) Calcium imaging of GCaMP6m in the cell bodies of R71G01-LexA labeled P1 neurons with P2X2-expressing DSK neurons activated by ATP. ATP: 2.5 mM. White dashed lines indicate ROI. Scale bars …
(A–C) Conditionally overexpression of Dsk increases number of lunges compared to controls. Pairs of flies of the indicated genotypes were introduced into one chamber, one of which overexpressed DSK …
Right, female aggression of wildtypes. Female ΔDsk mutants show fewer aggressive encounters than wildtypes.
Key resources table.
The number of labeled cells of mosaic flies.