Interplay of opposing fate choices stalls oncogenic growth in murine skin epithelium
Abstract
Skin epithelium can accumulate a high burden of oncogenic mutations without morphological or functional consequences. To investigate the mechanism of oncogenic tolerance, we induced HrasG12V in single murine epidermal cells and followed them long-term. We observed that HrasG12V promotes an early and transient clonal expansion driven by increased progenitor renewal that is replaced with an increase in progenitor differentiation leading to reduced growth. We attribute this dynamic effect to emergence of two populations within oncogenic clones: renewing progenitors along the edge and differentiating ones within the central core. As clone expansion is accompanied by progressive enlargement of the core and diminishment of the edge compartment, the intra-clonal competition between the two populations results in stabilized oncogenic growth. To identify the molecular mechanism of HrasG12V-driven differentiation, we screened known Ras-effector in vivo, and identified Rassf5 as a novel regulator of progenitor fate choice that is necessary and sufficient for oncogene-specific differentiation.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data file has been provided for Supplementary File 1.
Article and author information
Author details
Funding
National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR070780)
- Slobodan Beronja
Cell and Molecular Biology Training Grant (Graduate Student Fellowship)
- Madeline Sandoval
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal experimentation: Mice were housed and cared for in an AAALAC-accredited facility at Fred Hutchinson Cancer Research Center. All animal experiments were conducted under approved IACUC protocol number 50814 (approval date 12/01/2018-11/29/2021).
Copyright
© 2021, Sandoval et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.