Pattern regulation in a regenerating jellyfish
- ENS Lyon, France
- Sorbonne Université, France
- University of Vienna, Austria
Abstract
Clytia hemisphaerica jellyfish, with their tetraradial symmetry, offer a novel paradigm for addressing patterning mechanisms during regeneration. Here we show that an interplay between mechanical forces, cell migration and proliferation allows jellyfish fragments to regain shape and functionality rapidly, notably by efficient restoration of the central feeding organ (manubrium). Fragmentation first triggers actomyosin-powered remodeling that restores body umbrella shape, causing radial smooth muscle fibers to converge around 'hubs' which serve as positional landmarks. Stabilization of these hubs, and associated expression of Wnt6, depends on the configuration of the adjoining muscle fiber 'spokes'. Stabilized hubs presage the site of the manubrium blastema, whose growth is Wnt/β-catenin dependent and fueled by both cell proliferation and long-range cell recruitment. Manubrium morphogenesis is modulated by its connections with the gastrovascular canal system. We conclude that body patterning in regenerating jellyfish emerges mainly from local interactions, triggered and directed by the remodeling process.
Data availability
Transcriptomic data are being deposited in ENA (EBI) - accession code PRJEB37920. All other data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided.
Article and author information
Author details
Funding
Agence Nationale de la Recherche (ANR-13-PDOC-0016)
- Lucas Leclère
Agence Nationale de la Recherche (ANR-19-CE13-0003)
- Lucas Leclère
Fondation pour la Recherche Médicale (FDT201805005536)
- Sophie Peron
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Phillip A Newmark, Morgridge Institute for Research, United States
Publication history
- Received: March 24, 2020
- Accepted: September 5, 2020
- Accepted Manuscript published: September 7, 2020 (version 1)
- Version of Record published: September 29, 2020 (version 2)
Copyright
© 2020, Sinigaglia et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Pattern regulation in a regenerating jellyfish
eLife 9:e54868.
https://doi.org/10.7554/eLife.54868
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