Lipid accumulation controls the balance between surface connection and scission of caveolae
- Umeå University, Sweden
- Swedish University of Agricultural Sciences, Sweden
- Uppsala University, Sweden
Abstract
Caveolae are bulb-shaped invaginations of the plasma membrane (PM) that undergo scission and fusion at the cell surface and are enriched in specific lipids. However, the influence of lipid composition on caveolae surface stability is not well described or understood. Accordingly, we inserted specific lipids into the cell PM via membrane fusion and studied their acute effects on caveolae dynamics. We demonstrate that sphingomyelin stabilizes caveolae to the cell surface, while cholesterol and glycosphingolipids drive caveolae scission from the PM. Whilst all three lipids accumulated specifically in caveolae, cholesterol and sphingomyelin were actively sequestered, whereas glycosphingolipids diffused freely. The ATPase EHD2 restricts lipid diffusion and counteracts lipid-induced scission. We propose that specific lipid accumulation in caveolae generates an intrinsically unstable domain prone to scission if not restrained by EHD2 at the caveolae neck. This work provides a mechanistic link between caveolae and their ability to sense the PM lipid composition.
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All data generated or analysed during this study are included in the manuscript, supporting files and source data files provided for each figure
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Funding
Vetenskapsrådet (dnr 2017-04028)
- Richard Lundmark
Cancerfonden (CAN 2017/735)
- Richard Lundmark
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Hubert et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Lipid accumulation controls the balance between surface connection and scission of caveolae
eLife 9:e55038.
https://doi.org/10.7554/eLife.55038
