Viral-induced alternative splicing of host genes promotes influenza replication
Abstract
Viral infection induces the expression of numerous host genes that impact the outcome of infection. Here we show that infection of human lung epithelial cells with Influenza A virus (IAV) also induces a broad program of alternative splicing of host genes. While these splicing-regulated genes are not enriched for canonical regulators of viral infection, we find that many of these genes do impact replication of IAV. Moreover, in several cases, specific inhibition of the IAV-induced splicing pattern also attenuates viral infection. We further show that approximately a quarter of the IAV-induced splicing events are regulated by hnRNP K, a host protein required for efficient splicing of the IAV M transcript in nuclear speckles. Finally, we find an increase in hnRNP K in nuclear speckles upon IAV infection, which may alter accessibility of hnRNP K for host transcripts thereby leading to a program of host splicing changes that promote IAV replication.
Data availability
Sequencing data have been deposited in GEO under accession code GSE142499
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Viral-Induced Alternative Splicing of Host Genes Promotes Influenza ReplicationNCBI Gene Expression Omnibus GSE142499.
Article and author information
Author details
Funding
National Institutes of Health (R35 GM118048)
- Matthew G Thompson
- Michael J Mallory
- Max B Ferretti
- Kristen W Lynch
National Institutes of Health (R01 AI125524)
- Matthew G Thompson
- Prasanna Bhat
- Beatriz MA Fontoura
- Kristen W Lynch
National Institutes of Health (R01 AI150246,R01 AI122749,R01 AI140539)
- Mark Dittmar
- Sara Cherry
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Thompson et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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