A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes.
- James T Kadonaga
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Wolf-Dietrich Heyer, University of California, Davis, United States
© 2020, Cruz-Becerra & Kadonaga
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