Srsf10 and the minor spliceosome control tissue-specific and dynamic SR protein expression

Abstract
Data availability
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Abstract

Minor and major spliceosomes control splicing of distinct intron types and are thought to act largely independent of one another. SR proteins are essential splicing regulators mostly connected to the major spliceosome. Here, we show that Srsf10 expression is controlled through an autoregulated minor intron, tightly correlating Srsf10 with minor spliceosome abundance across different tissues and differentiation stages in mammals. Surprisingly, all other SR proteins also correlate with the minor spliceosome and Srsf10, and abolishing Srsf10 autoregulation by Crispr/Cas9-mediated deletion of the autoregulatory exon induces expression of all SR proteins in a human cell line. Our data thus reveal extensive crosstalk and a global impact of the minor spliceosome on major intron splicing.

Data availability

We have only used data that are publically available.

The following previously published data sets were used

1. Tanikawa et al
(2017) p53 mouse multi-tissue transcriptome analysis
SRA, DRP003641.

https://www.ncbi.nlm.nih.gov/sra/?term=DRP003641
1. Hubbard et al
(2012) Deep transcriptional profiling of longitudinal changes during neurogenesis and network maturation in vivo
SRA, SRP017778.

https://www.ncbi.nlm.nih.gov/sra/?term=SRP017778
1. Uhlen et al
(2015) Tissue-based map of the human proteome
Science, 10.1126/science.1260419.

https://science.sciencemag.org/content/suppl/2015/01/21/347.6220.1260419.DC1

Article and author information

Author details

Stefan Meinke

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-5083-3383
Gesine Goldammer

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

Competing interests
The authors declare that no competing interests exist.
A Ioana Weber

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

Competing interests
The authors declare that no competing interests exist.
Victor Tarabykin

Institute for Cell and Neurobiology, Charité-Universitätsmedizin Berlin, Berlin, Germany

Competing interests
The authors declare that no competing interests exist.
Alexander Neumann

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

Competing interests
The authors declare that no competing interests exist.
Marco Preussner

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

For correspondence
mpreussner@zedat.fu-berlin.de

Competing interests
The authors declare that no competing interests exist.
Florian Heyd

Institute of Chemistry and Biochemistry, FU Berlin, Berlin, Germany

For correspondence
florian.heyd@fu-berlin.de

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-9377-9882

Funding

Deutsche Forschungsgemeinschaft (278001972 - TRR 186)

Florian Heyd

Deutsche Forschungsgemeinschaft (TA303/8-1)

Victor Tarabykin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Colonies of wild type mice of the NMRI strain were maintained in the animal facilities of Charité-Universitätsmedizin Berlin. Tissue collection was performed in compliance with German Animal Welfare Law and regulations imposed by the State Office for Health and Social Affairs Council in Berlin / Landesamt für Gesundheit und Soziales (LAGeSo) under licence T102/11.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.