Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
- University of Illinois at Chicago, United States
- Cornell University, United States
Abstract
Adipocytes arise from distinct progenitor populations during development and adult, but little is known about how developmental progenitors differ from adult progenitors. Here, we investigate the role of platelet-derived growth factor receptor alpha (PDGFRα) in the divergent regulation of the two different adipose progenitor cells (APCs). Using in vivo adipose lineage tracking and deletion mouse models, we found that developmental PDGFRα+ cells are adipogenic and differentiated into mature adipocytes, and the deletion of Pdgfra in developmental adipose lineage disrupted white adipose tissue (WAT) formation. Interestingly, adult PDGFRα+ cells do not significantly contribute to adult adipogenesis, and deleting Pdgfra in adult adipose lineage did not affect WAT homeostasis. Mechanistically, embryonic APCs require PDGFRα for fate maintenance, and without PDGFRα, they underwent fate change from adipogenic to fibrotic lineage. Collectively, our findings indicate that PDGFRα+ cells and Pdgfra gene itself are differentially required for WAT development and adult WAT homeostasis.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
NIDDK (K01 DK111771)
- Yuwei Jiang
NIDDK DRTC (P30DK020595)
- Yuwei Jiang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Valerie Horsley, Yale University, United States
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (#18-184) of the University of Illinois at Chicago. The protocol was reviewed in accordance with the Animal Care Policies and Procedures of the University of Illinois at Chicago and renewed on 10/16/2019. All experimental animals will be euthanized by carbon dioxide gas inhalation in accordance with the guidelines of the American Veterinary Medical Association and the policies of the UIC IACUC.
Version history
- Received: February 20, 2020
- Accepted: June 18, 2020
- Accepted Manuscript published: June 19, 2020 (version 1)
- Version of Record published: July 6, 2020 (version 2)
Copyright
© 2020, Shin et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
eLife 9:e56189.
https://doi.org/10.7554/eLife.56189
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