(A) Traumatic injury of the sciatic nerve (CCI, green) in Wistar rats results in mechanical allodynia after one week as measured by von Frey Hairs (paw withdrawal threshold) in comparison to the contralateral side (grey). (B, C) Quantification of Cirl1 (B) and Cirl3 (C) mRNA levels in subpopulations of rat DRG neurons via in situ hybridization (RNAscope). Shown are control conditions (naïve DRGs, grey) and one week after injury (green) following the emergence of allodynia. Data are presented as mean and individual values. Asterisks denote level of significance, **p≤0.01, ***p≤0.001. (D, E) Example images of the RNAscope assay in DRG neurons. Shown are projections of confocal stacks stained against IB4 and labelled with probes against Cirl1 and Cirl3 under control conditions (D) and CCI (E). Scale bar 20 µm.