8- to 12-week-old C57BL/6J mice were ad libitum-fed (non-fasted) or fasted for 24 hr (fasted). (A–D) Hepatic proteins were measured by immunoblotting whole-cell lysates (A; quantified results are …
(A) Gluconeogenic genes. (B) TAZ target gene involved in proliferation.
Immunohistochemistry using an anti-TAZ antibody in L-TAZ KO and control floxed (Flox) mouse liver sections (CV, central vein; PV, portal vein; scale bar, 50 μM).
Primary mouse hepatocytes were incubated in low glucose media (1 g/L) without fetal bovine serum (FBS) for overnight and then placed in high glucose media (4.5 g/L) with FBS for the indicated times. …
(A–G) 8- to 12-week-old C57BL/6J mice were administered AdshTAZ or AdshControl (AdshCon), then sacrificed 8 days later in the ad libitum-fed state, when the mRNA expression of hepatic genes (A, C) …
Body (A), epididymal white adipose tissue (WAT) (B), and liver weight (C) were measured. (D) H&E staining of liver sections (scale bar, 50 μM). Data were analyzed by unpaired Student’s t-test; **p<0.…
Hepatic proteins were measured by immunoblotting whole-cell lysates.
Plasma insulin (A) and glucagon (B) were measured. Data were analyzed by unpaired Student’s t-test.
Body (A) and liver weight (B) were measured. (C) H&E staining of liver sections (scale bar, 50 μM). Data were analyzed by two-way ANOVA (A) and unpaired Student’s t-test (B).
(A) Hepatic proteins were measured by immunoblotting whole-cell lysates. (B, C) Insulin sensitivity was assessed by intraperitoneal insulin tolerance test (ITT). Glucose percentages relative to the …
8- to 12-week-old C57BL/6J mice were administered AdGFP or AdTAZ, then sacrificed 5 days later, after a 24 hr fast, when the hepatic protein levels in nuclear extracts (A [representative image, left;…
CV, central vein; PV, portal vein; scale bar, 500 μM.
Mouse body weight (A), epididymal white adipose tissue weight (WAT) weight (B), liver weight (C), and plasma insulin (F, left) and glucagon (F, right) levels were measured. (D) H&E staining of liver …
Primary mouse hepatocytes were isolated from 8- to 12-week-old C57BL/6J mice. (A–D) To knock down TAZ, cells were infected with AdshTAZ or AdshControl (AdshCon). (E–H) To overexpress TAZ, cells were …
Primary mouse hepatocytes were infected with AdTAZ or control AdGFP and were treated with Dex (100 nM) for 6 hr. Gene expression was measured using real-time RT-PCR. Data were analyzed by two-way …
Cells were infected with AdshTAZ or AdshCon (A) or AdTAZ or AdGFP (B). Protein levels were measured by immunoblotting whole-cell lysates.
Cells were infected with AdshTAZ or AdshCon (A, B) or AdTAZ or AdGFP (C, D). Protein levels were measured by immunoblotting whole-cell lysates. Cells were treated with glucagon (20 nM) for 30 min (A,…
(A–D, G, K, L, N, O) HepG2 cells were co-transfected with expression vectors, luciferase reporters, and an internal control (Renilla), and treated with dexamethasone (Dex; 100 nM), as indicated. …
A conserved I/LPKY motif is identified in glucocorticoid receptor (GR) from various species.
Highlighted in red.
(A, C) HepG2 cells were co-transfected with expression vectors, luciferase reporters, and an internal control (Renilla), and treated with dexamethasone (Dex) (100 nM), as indicated. Relative …
293A A cells were co-transfected with expression vectors, luciferase reporters, and an internal control (Renilla), and treated with dexamethasone (Dex) (100 nM), as indicated. Relative luciferase …
(A) 293A A cells were transfected with expression vectors for GR fused with a GFP, TAZ, or a control empty vector (Con), and treated with dexamethasone (Dex) (100 nM) or vehicle for 6 hr prior to …
(A, G) Endogenous TAZ was immunoprecipitated from liver nuclear extracts prepared from ad libitum-fed C57BL/6J mice (A) or mice treated with RU486 or vehicle (G), and the amounts of GR in the …
8- to 12-week-old male C57BL/6J mice were administered adenoviruses expressing TAZ (flag-tagged) or GFP for 5 days. Chromatin immunoprecipitation (ChIP) assays were performed from liver extracts …
8- to 12-week-old C57BL/6J J mice were administered adenoviruses expressing TAZ or GFP for 5 days. Mice were treated with Dex or vehicle. Blood glucose (A) and hepatic gene expression (B) were …
C57BL/6J mice (A–D) or (E) C57BL/6J mice were ad libitum-fed (non-fasted) or fasted for 24 hr. (A) Blood glucose. (B) Pyruvate tolerance testing (PTT). (C) Hepatic gene expression. (D, E) Chromatin …
Mice were administered adenoviruses expressing TAZ mutants (flag-tagged) or GFP for 5 days. Body weight (A) and liver weight (B) were measured, and hepatic protein levels (C) were measured by …
(A–C) 8- to 10-week-old L-DKO or flox controls were administered the indicated adenoviruses. (A) Blood glucose. (B) Pyruvate tolerance testing (PTT). (C) Hepatic gene expression. (D) 4- to …
(A) Hepatic proteins were measured by immunoblotting nuclear (Nuc) or cytoplasmic (Cyto) extracts of L-DKO and flox control mice. (B, C) Female L-DKO and flox controls were administered adenoviruses …
Figures 2G (A), 2L (C), and 2M (E), which are plotted using SEM, are compared with Figures R1B, R1D, and R1F, respectively, which are plotted using SD.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Mus musculus) | C57BL/6J | Jackson Laboratory | Stock #: 000664 RRID:IMSR_JAX:000664 | |
Genetic reagent (M. musculus) | Tazflox/flox: Yapflox/flox | Jackson Laboratory | Stock #: 030532RRID:IMSR_JAX:030532 | |
Genetic reagent (M. musculus) | Albumin-Cre | Jackson Laboratory | Stock #: 003574RRID:IMSR_JAX:003574 | |
Genetic reagent (M. musculus) | Tazflox/flox: Alb-Cre | This paper | See ‘Animals and treatments’ in Materials and methods | |
Cell line (Homo sapiens) | HepG2 | ATCC | Cat. #: HB-8065RRID:CVCL 0027 | |
Cell line (H. sapiens) | 293A | Thermo Fisher Scientific | Cat. #: R70507RRID:CVCL_6910 | |
Commercial assay or kit | BLOCK-iT U6 RNAi entry vector kit | Thermo Fisher Scientific | Cat. #: K494500 | |
Commercial assay or kit | BLOCK-iT U6 adenoviral RNAi expression system | Thermo Fisher Scientific | Cat. #: K494100 | |
Commercial assay or kit | Stellux Chemiluminscence rodent insulin ELISA kit | Alpco | Cat. #:80-INSMR-CH01 | |
Commercial assay or kit | Mouse glucagon ELISA kit | Alpco | Cat. #:48-GLUHU-E01 | |
Commercial assay or kit | Dual-luciferase reporter assay kit | Promega | Cat. #: E1960 | |
Commercial assay or kit | VIP substrate Kit, HRP | Vector Laboratories | Cat. #: SK-4600 RRID:AB_2336848 | PMID:28123024 |
Commercial assay or kit | Mutagenesis kit | Agilent | Cat. #: 210,519 | |
Commercial assay or kit | cDNA synthesis kit | Thermo Fisher Scientific | Cat. #: 4368813 | |
Commercial assay or kit | NE-PER Nuclear and Cytoplasmic Extraction kit | Thermo Fisher Scientific | Cat. #: 78835 | |
Commercial assay or kit | Amplex glucose oxidase assay kit | Thermo Fisher Scientific | Cat. #: A22189 | |
Recombinant DNA reagent | pCMV-TOPO TAZ (human) | Addgene | Cat. #: 24809 RRID:Addgene_24809 | PMID:18568018 |
Recombinant DNA reagent | pcDNA3-flag-TAZ (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pcDNA3-flag-TAZ S51A (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pcDNA3-flag-TAZ S89A (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pCMV-TOPO TAZΔWW (human) | Addgene | Cat. #: 24811RRID:Addgene_24811 | PMID:18568018 |
Recombinant DNA reagent | pCMV-TOPO TAZΔCC (human) | Addgene | Cat. #: 24816 RRID:Addgene_24816 | PMID:18568018 |
Recombinant DNA reagent | pcDNA3-flag-TAZΔWW (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pcDNA3-flag- TAZΔCC (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pcDNA3-flag-TAZ WW | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pGL3-3XGRE-Luc | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pGL3-G6PC-Luc (human) | Dr. Pere Puigserver | ||
Recombinant DNA reagent | pGL3-PCK1-Luc (human) | Dr. Pere Puigserver | ||
Recombinant DNA reagent | pcDNA3-HNF4 α (mouse) | Dr. Pere Puigserver | ||
Recombinant DNA reagent | pcDNA3-PGC1α (mouse) | Dr. Pere Puigserver | ||
Recombinant DNA reagent | pEGFP-GR | Addgene | Cat. #: 47504 RRID:Addgene_47504 | |
Recombinant DNA reagent | pcDNA3-GR (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pcDNA3-GR4A (human) | This paper | See ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | 8xGTIIC-Luc | Addgene | Cat. #: 34615 RRID:Addgene_34615 | PMID:21654799 |
Recombinant DNA reagent | pcDNA3-Flag-YAP1 (human) | Addgene | Cat. #: 18881 RRID:Addgene_18881 | PMID:18280240 |
Recombinant DNA reagent | pRK5-TEAD1 (human) | Addgene | Cat. #: 33109 RRID:Addgene_33109 | PMID:18579750 |
Recombinant DNA reagent | pAd-Track-CMV-GFP | Addgene | Cat. #: 16405 RRID:Addgene_16405 | PMID:9482916Construct to establish adenovirus |
Recombinant DNA reagent | pAd-Track-CMV-Flag-TAZ (human) | This paper | Construct to establish adenovirus expressing TAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pAd-Track-CMV-Flag-TAZΔWW (human) | This paper | Construct to establish adenovirus expressing TAZΔWW; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | pAd-Track-CMV-Flag-TAZS89A (human) | This paper | Construct to establish adenovirus expressing TAZS89A; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | U6-shLamin (human) | This paper | Control for shTAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | U6-shTAZ (human) | This paper | Construct to knockdown TAZ in HepG2 cells; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | U6-shLacZ | This paper | Construct to establish adenovirus expressing shControl; control for shTAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | U6-shTAZ (mouse) | This paper | Construct to establish adenovirus expressing shTAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-shLacZ | This paper | Control adenovirus expressing shLacZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-shTAZ (mouse) | This paper | Adenovirus expressing shTAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-Track-CMV-GFP | This paper | Control adenovirus expressing GFP, generated from pAd-Track-CMV-GFP vector; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-Track-CMV-flag-TAZ | This paper | Adenovirus generated from pAd-Track-CMV-TAZ; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-Track-CMV-flag-TAZΔWW | This paper | Adenovirus generated from pAd-Track-CMV-TAZΔWW; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Recombinant DNA reagent | Ad-Track-CMV-flag-TAZS89A | This paper | Adenovirus generated from pAd-Track-CMV-TAZS89A; see ‘Plasmid and adenoviral vector constructs’ in Materials and methods | |
Sequence-based reagent | shLamin (human) | This paper | CTGGACTTCCAGAAGAACA | |
Sequence-based reagent | shLacZ | This paper | CTACACAAATCAGCGATTT | |
Sequence-based reagent | shTAZ (human) | This paper | GCTCAGATCCTTTCCTCAATG | |
Sequence-based reagent | shTAZ (mouse) | This paper | GCCAGAGATACTTCCTTAATC | |
Sequence-based reagent | Mouse-Tbp-F | This paper | qRT-PCR primer | ACCTTCACCAATGACTCCTATG |
Sequence-based reagent | Mouse-Tbp-R | This paper | qRT-PCR primer | TGACTGCAGCAAATCGCTTGG |
Sequence-based reagent | Mouse-Cry61-F | This paper | qRT-PCR primer | CAAGAAATGCAGCAAGACCA |
Sequence-based reagent | Mouse-Cry61-R | This paper | qRT-PCR primer | GGCCGGTATTTCTTGACACT |
Sequence-based reagent | Mouse-Ctgf-F | This paper | qRT-PCR primer | TCCACCCGAGTTACCAATGA |
Sequence-based reagent | Mouse-Ctgf -R | This paper | qRT-PCR primer | CAAACTTGACAGGCTTGGC |
Sequence-based reagent | Mouse-G6pc-F | This paper | qRT-PCR primer | TGGCTTTTTCTTTCCTCGAA |
Sequence-based reagent | Mouse-Pck1-F | This paper | qRT-PCR primer | TCGGAGACTGGTTCAACCTC |
Sequence-based reagent | Mouse-Pck1-R | This paper | qRT-PCR primer | GAGGGACAGCAGCACCAT |
Sequence-based reagent | Mouse-Taz-F | This paper | qRT-PCR primer | ACAGGTGAAAATTCCGGTCA |
Sequence-based reagent | Mouse-Taz -R | This paper | qRT-PCR primer | GAAGGCAGTCCAGGAAATCA |
Sequence-based reagent | Mouse-Yap-F | This paper | qRT-PCR primer | AAGCCATGACTCAGGATGGA |
Sequence-based reagent | Mouse-Yap-R | This paper | qRT-PCR primer | GTTCATGGCAAAACGAGGGTC |
Sequence-based reagent | Mouse-Ctgf-ChIP-F | This paper | qChIP primer | TTCCTGGCGAGCTAAAGTGT |
Sequence-based reagent | Mouse-Ctgf-ChIP-R | This paper | qChIP primer | CCTTCCTGCCTCATCAACTC |
Sequence-based reagent | Mouse-G6pc-ChIP-GRE-F | This paper | qChIP primer | AGCACTGTCAAGCAGTGTGC |
Sequence-based reagent | Mouse-G6pc-ChIP-GRE-F | This paper | qChIP primer | GCAAAACAGGCACACAAAAA |
Sequence-based reagent | Mouse-G6pc-ChIP-HNF4E-F | This paper | qChIP primer | CCCTGAACATGTTTGCATCA |
Sequence-based reagent | Mouse-G6pc-ChIP-HNF4E-R | This paper | qChIP primer | GTAGGTCAATCCAGCCCTGA |
Sequence-based reagent | Mouse-Pck1-ChIP-Con-F | This paper | qChIP primer | TGGGAGACACACATCTTATTCCA |
Sequence-based reagent | Mouse-Pck1-ChIP-Con-R | This paper | qChIP primer | GTCCCTCTATAGACTTCCAGCACA |
Sequence-based reagent | Mouse-Pck1-ChIP-GRE-F | This paper | qChIP primer | TGCAGCCAGCAACATATGAA |
Sequence-based reagent | Mouse-Pck1-ChIP-GRE-F | This paper | qChIP primer | TGATGCAAACTGCAGGCTCT |
Sequence-based reagent | Mouse-Pck1-ChIP-HNF4E-F | This paper | qChIP primer | TAAGGCAAGAGCCTGCAGTT |
Sequence-based reagent | Mouse-Pck1-ChIP-HNF4E-F | This paper | qChIP primer | AGGCCCCTCTATCAGCCATA |
Antibody | (Rabbit polyclonal) anti-TAZ | Cell Signaling Technology | Cat. #: 4883 RRID:AB_1904158 | PMID:29533785IB (1:1000) |
Antibody | (Rabbit monoclonal) anti-p-TAZ (Ser89) | Cell Signaling Technology | Cat. #: 59971 RRID:AB_2799578 | IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-AKT | Cell Signaling Technology | Cat. #: 9272 RRID:AB_329827 | PMID:23653460IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-p-AKT (Thr308) | Cell Signaling Technology | Cat. #: 9275 RRID:AB_329828 | PMID:23715867IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-p-AKT (Ser473) | Abclonal | Cat. #: AP0098 RRID:AB_2770899 | IB (1:1000) |
Antibody | (Mouse monoclonal) anti-beta-actin | Santa Cruz Biotechnology | Cat. #: sc-47778 RRID:AB_2714189 | PMID:28017329IB (1:3000) |
Antibody | (Rabbit monoclonal) anti-CREB | Cell Signaling Technology | Cat. #: 9197RRID:AB_331277 | PMID:24080368IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-p-CREB (Ser133) | Abclonal | Cat. #: AP0333RRID:AB_2771008 | IB (1:1000) |
Antibody | (Mouse monoclonal) anti-Flag | Abclonal | Cat. #: AE005RRID:AB_2770401 | IB (1:10000)IP (1 µg/IP)ChIP (1–2 µg/IP) |
Antibody | (Rabbit polyclonal) anti-Flag | Cell Signaling Technology | Cat. #: 2368 RRID:AB_2217020 | PMID:25514086IB (1:1000) |
Antibody | (Rabbit monoclonal) anti-FoxO1 | Cell Signalling Technology | Cat. #: 2880 RRID:AB_2106495 | PMID:24248465IB (1:1000) |
Antibody | (Rabbit monoclonal) anti-p-FoxO1 (Ser256) | Cell Signaling Technology | Cat. #: 84192 RRID:AB_2800035 | PMID:31583122IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-G6PC | Abcam | Cat. #: ab83690 RRID:AB_1860503 | PMID:25774555IB (1:1000) |
Antibody | (Mouse monoclonal) anti-GAPDH | Santa Cruz Biotechnology | Cat. #: sc-32233 RRID:AB_627679 | PMID:24105481IB (1:1000) |
Antibody | (Mouse monoclonal) anti-GLUL | BD Biosciences | Cat. #: 610517 RRID:AB_397879 | PMID:17120293IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-GR | Abclonal | Cat. #: A2164 RRID:AB_2764182 | IB (1:3000) |
Antibody | Goat polyclonal anti-HMGCR | Santa Cruz Biotechnology | Cat. #: sc-27578 RRID:AB_2118199 | PMID:26824363IB (1:1000) |
Antibody | (Rabbit polyclonal) anti-HNF4α | Santa Cruz Biotechnology | Cat. #: sc-8987 RRID:AB_2116913 | PMID:29937200IB (1:000) |
Antibody | (Rabbit polyclonal) anti-PGC1α | Abclonal | Cat. #: A12348 RRID:AB_2759191 | IB (1:000) |
Antibody | (Rabbit monoclonal) anti-Tubulin | Cell SignalingTechnology | Cat. #: 2125 RRID:AB_2619646 | PMID:28343940IB (1:5000) |
Antibody | (Mouse monoclonal) anti-Vinculin | Santa Cruz Biotechnology | Cat. #: sc-73614 RRID:AB_1131294 | PMID:29017056IB (1:5000) |
Antibody | (Rabbit polyclonal) anti-YAP | Cell Signaling Technology | Cat. #: 4912 RRID:AB_2218911 | PMID:28323616IB (1:000) |
Antibody | (Mouse polyclonal) anti-IRS1 | Provided by Dr. Morris White | PMID:29867232IB (1:000) | |
Antibody | (Mouse polyclonal) anti-IRS2 | Provided by Dr. Morris White | PMID:29867232IB (1:000) | |
Antibody | (Rabbit polyclonal) anti-Ac-Histone4 | Abclonal | Cat. #: A15233 RRID:AB_2762128 | ChIP (1 µg/IP) |
Antibody | (Mouse monoclonal) anti-GR | Santa Cruz Biotechnology | Cat. #: Sc-393232 RRID:AB_2687823 | PMID:28467930ChIP (1–2 µg/IP) |
Antibody | (Rabbit polyclonal) anti-TAZ | Abclonal | Cat. #: A8202 RRID:AB_2721146 | ChIP (1–2 µg/IP)IHC (1: 200) |
Antibody | Goat anti-(Rabbit polyclonal) IgG-HPR | Thermo Fisher Scientific | Cat. #: 31460RRID:AB_228341 | PMID:24932808IB (1:5000–20,000) |
Antibody | Goat anti-(Mouse polyclonal) IgG-HRP | Thermo Fisher Scientific | Cat. #: 31430RRID:AB_228307 | PMID:10359649IB (1:5000–20,000) |
Antibody | Rabbit anti-goat (Rabbit polyclonal) IgG-HRP | Santa Cruz Biotechnology | Cat. #: sc-2768RRID:AB_656964 | PMID:23970784IB (1:5000–15,000) |
Chemical compound, drug | Glucagon | Sigma | Cat. #: G2044 | |
Chemical compound, drug | RU486 | Sigma | Cat. #: M8046 | |
Chemical compound, drug | Dexamethasone | Sigma | Cat. #: D4902 | For cell culture studies |
Chemical compound, drug | Dexamethasone | Sigma | Cat. #: 2915 | For in vivo studies |
Chemical compound, drug | Sodium pyruvate | Sigma | Cat. #: P5280 | |
Chemical compound, drug | Protease inhibitor cocktail tablet | Sigma | Cat. #: S8820 | |
Chemical compound, drug | Phosphatase inhibitor cocktail tablet | Sigma | Cat. #: 4906837001 | |
Chemical compound, drug | Bovine insulin | Sigma | Cat. #: I0516 | For cell culture studies |
Chemical compound, drug | Human insulinHumulin R U-100 | Eli Lily | Cat. #: HI-210 | For in vivo studies |
Chemical compound, drug | Percoll | Cytiva | Cat. #: 17089109 | |
Chemical compound, drug | Trizol | Thermo Fisher Scientific | Cat. #: 15596018 | |
Chemical compound, drug | DSP | Thermo Fisher Scientific | Cat. #: PG82081 | |
Other | SYBR Green PCR master mix | Bioline | Cat. #: BIO-84050 | |
Other | Collagen Type I Rat Tail | Corning | Cat. #: 354,236 | |
Other | Collagenase Type I | Worthington Biochemical Corporation | Cat. #: LS004196 | |
Other | PVDF membrane | Sigma | Cat. #: IPVH00010 | |
Other | ECL | Thermo Fisher Scientific | Cat. #: A43841 | |
Other | Agarose A/G beads | Santa Cruz Biotechnology | Cat. #: sc-2003 RRID:AB_10201400 | PMID:28392145 |
Other | DMEM cell culture media | Thermo Fisher Scientific | Cat. #: 11965118 | |
Other | M199 cell culture media | Thermo Fisher Scientific | Cat. #:11043023 | |
Other | DMEM low glucose cell culture media, no phenol red | Thermo Fisher Scientific | Cat. #:11054020 | |
Other | Lipofectamine 2000 | Thermo Fisher Scientific | Cat. #: 11668019 | Transfection reagent |
Time (min) | ||||||
---|---|---|---|---|---|---|
Treatment | 0 | 15 | 30 | 60 | 120 | |
AdshCon | 76 | 91 | 120 | 105 | 73 | |
AdshCon | 73 | 116 | 139 | 119 | 64 | |
AdshCon | 76 | 109 | 100 | 86 | 71 | |
AdshCon | 67 | 93 | 98 | 103 | 72 | |
AdshCon | 63 | 119 | 119 | 104 | 57 | |
AdshCon | 78 | 104 | 120 | 83 | 68 | |
AdshCon | 66 | 96 | 122 | 106 | 67 | |
AdshTAZ | 96 | 129 | 161 | 168 | 91 | |
AdshTAZ | 79 | 130 | 149 | 114 | 103 | |
AdshTAZ | 80 | 122 | 135 | 116 | 105 | |
AdshTAZ | 105 | 195 | 195 | 116 | 108 | |
AdshTAZ | 86 | 140 | 162 | 146 | 94 | |
AdshTAZ | 85 | 130 | 155 | 122 | 89 | |
AdshTAZ | 102 | 129 | 127 | 125 | 94 | |
Ave | AdshCon | 71.3 | 104.0 | 116.9 | 100.9 | 67.4 |
AdshTAZ | 90.4 | 139.3 | 154.9 | 129.6 | 97.7 | |
SEM | AdshCon | 2.222 | 4.220 | 5.302 | 4.698 | 2.103 |
AdshTAZ | 3.981 | 9.496 | 8.316 | 7.615 | 2.826 | |
SD | AdshCon | 5.880 | 11.165 | 14.029 | 12.429 | 5.563 |
AdshTAZ | 10.533 | 25.124 | 22.003 | 20.148 | 7.477 | |
Two-way ANOVA | p | 0.0942 | 0.0002 | <0.0001 | 0.0033 | 0.0017 |
Time (min) | ||||||
---|---|---|---|---|---|---|
Treatment | 0 | 15 | 30 | 60 | 120 | |
Flox (F) | 56 | 71 | 75 | 63 | 34 | |
Flox (F) | 44 | 85 | 98 | 70 | 60 | |
Flox (F) | 52 | 79 | 103 | 86 | 51 | |
Flox (F) | 54 | 89 | 97 | 63 | 52 | |
Flox (F) | 51 | 82 | 103 | 85 | 47 | |
Flox (F) | 52 | 89 | 101 | 76 | 53 | |
Flox (F) | 53 | 87 | 89 | 83 | 47 | |
Flox (F) | 54 | 81 | 95 | 66 | 59 | |
L-TAZ KO (F) | 58 | 97 | 120 | 86 | 63 | |
L-TAZ KO (F) | 66 | 102 | 114 | 86 | 73 | |
L-TAZ KO (F) | 58 | 89 | 106 | 91 | 59 | |
L-TAZ KO (F) | 57 | 93 | 111 | 83 | 64 | |
L-TAZ KO (F) | 56 | 93 | 108 | 88 | 62 | |
L-TAZ KO (F) | 58 | 96 | 109 | 87 | 53 | |
L-TAZ KO (F) | 57 | 89 | 105 | 96 | 65 | |
L-TAZ KO (F) | 63 | 94 | 108 | 88 | 59 | |
L-TAZ KO (F) | 62 | 92 | 128 | 106 | 63 | |
L-TAZ KO (F) | 62 | 91 | 113 | 102 | 60 | |
Ave | Flox (F) | 52.0 | 82.9 | 95.1 | 74.0 | 50.4 |
L-TAZ KO (F) | 59.7 | 93.4 | 112.2 | 91.3 | 62.1 | |
SEM | Flox (F) | 1.268 | 2.142 | 3.308 | 3.464 | 2.890 |
L-TAZ KO (F) | 1.044 | 1.343 | 2.240 | 2.399 | 1.629 | |
SD | Flox (F) | 3.586 | 6.058 | 9.357 | 9.798 | 8.176 |
L-TAZ KO (F) | 3.302 | 4.248 | 70.84 | 7.587 | 5.152 | |
Two-way ANOVA | p | 0.0836 | 0.0067 | <0.0001 | <0.001 | 0.002 |
Time (min) | ||||||
---|---|---|---|---|---|---|
Treatment | 0 | 15 | 30 | 60 | 120 | |
Flox (M) | 46 | 65 | 102 | 69 | 52 | |
Flox (M) | 44 | 84 | 97 | 73 | 51 | |
Flox (M) | 51 | 89 | 95 | 79 | 56 | |
Flox (M) | 54 | 78 | 98 | 69 | 57 | |
Flox (M) | 53 | 74 | 101 | 77 | 60 | |
Flox (M) | 46 | 82 | 108 | 77 | 56 | |
Flox (M) | 54 | 75 | 97 | 68 | 55 | |
Flox (M) | 51 | 89 | 103 | 79 | 58 | |
L-TAZ KO (M) | 56 | 92 | 129 | 85 | 62 | |
L-TAZ KO (M) | 56 | 94 | 118 | 95 | 59 | |
L-TAZ KO (M) | 59 | 97 | 108 | 88 | 60 | |
L-TAZ KO (M) | 66 | 105 | 110 | 102 | 78 | |
L-TAZ KO (M) | 55 | 106 | 110 | 92 | 66 | |
L-TAZ KO (M) | 61 | 93 | 115 | 92 | 68 | |
L-TAZ KO (M) | 58 | 99 | 105 | 87 | 71 | |
Ave | Flox (M) | 49.9 | 79.5 | 100.1 | 73.9 | 55.6 |
L-TAZ KO (M) | 58.7 | 98.0 | 113.6 | 91.6 | 66.3 | |
SEM | Flox (M) | 1.407 | 2.897 | 1.493 | 1.663 | 1.051 |
L-TAZ KO (M) | 1.443 | 2.138 | 3.046 | 2.170 | 2.552 | |
SD | Flox (M) | 3.980 | 8.194 | 4.224 | 4.704 | 2.973 |
L-TAZ KO (M) | 3.817 | 5.657 | 8.059 | 5.740 | 6.751 | |
Two-way ANOVA | p | 0.0173 | <0.0001 | <0.0001 | <0.0001 | <0.0026 |