(A) ATP3 mRNA proportion of association with mitochondria was measured in WT, sch9∆ and reg1∆ mutant strains, and upon chloramphenicol addition (WT + Chlo.) (n > 27). Error bar represents s.e.m. (B-F) Mitochondrial volume fraction (B), cell volume (C), mitochondrial volume (D), adjusted mitochondrial volume fraction (E), and vacuole volume fraction (F) in WT, mutant, and chloramphenicol conditions (n > 27). (G) Predicted relationship of proportion of mRNA localization to mitochondria and non-adjusted mitochondrial volume fraction from mathematical modeling of WT strains was plotted as green dotted lines. Mean value of each axis of WT, mutants cells, and cells from chloramphenicol added conditions were plotted as cross. Error bar represents s.e.m. (H, I) Relationship of the proportion of ATP3 mRNA localization to mitochondria and mitochondrial volume fraction from mathematical modeling. (I) Mitochondrial volume fraction was adjusted with effective cytoplasm volume. Green lines represent the relationship in WT cells, orange, blue, and grey lines represent sch9∆ and reg1∆ mutant cells, and chloramphenicol added condition respectively. Lines were plotted as described in Materials and methods.