Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit
- University of California, San Francisco, United States
- Stanford University, United States
- Gladstone Institute of Neurological Disease, United States
- University of California, San Francisco, United States
Abstract
We tested the proposal that the C-terminal domain (CTD) of the AMPAR subunit GluA1 is required for LTP. We found that a knock-in mouse lacking the CTD of GluA1 expresses normal LTP and spatial memory, assayed by the Morris water maze. Our results support a model in which LTP generates synaptic slots, which capture passively diffusing AMPARs.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Javier Díaz-Alonso
Funding
National Institute of Mental Health (K99MH118425)
- Javier Díaz-Alonso
National Institute of Mental Health (R01MH070957)
- Roger A Nicoll
National Institute of Mental Health (R01MH117139)
- Roger A Nicoll
National Institute of Mental Health (P50MH086403)
- Robert C Malenka
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: The authors declare that this study has been performed strictly following all relevant laboratory animal use regulations according to approved institutional animal care and use committee (IACUC) protocols of the University of California, San Francisco (AN170318 and AN183289), and Stanford University (10322).
Reviewing Editor
- Linda Overstreet-Wadiche, University of Alabama at Birmingham, United States
Version history
- Received: April 18, 2020
- Accepted: August 21, 2020
- Accepted Manuscript published: August 24, 2020 (version 1)
- Version of Record published: September 18, 2020 (version 2)
Copyright
© 2020, Díaz-Alonso et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit
eLife 9:e58042.
https://doi.org/10.7554/eLife.58042
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