The manifold actions of signaling peptides on subcellular dynamics of a receptor specify stomatal cell fate
- Unversity of Washington, United States
- Nagoya University, Japan
- University of Texas at Austin, United States
- University of Washington, United States
Abstract
Receptor endocytosis is important for signal activation, transduction, and deactivation. However, how a receptor interprets conflicting signals to adjust cellular output is not clearly understood. Using genetic, cell biological, and pharmacological approaches, we report here that ERECTA-LIKE1 (ERL1), the major receptor restricting plant stomatal differentiation, undergoes dynamic subcellular behaviors in response to different EPIDERMAL PATTERNING FACTOR (EPF) peptides. Activation of ERL1 by EPF1 induces rapid ERL1 internalization via multivesicular bodies/late endosomes to vacuolar degradation, whereas ERL1 constitutively internalizes in the absence of EPF1. The co-receptor, TOO MANY MOUTHS is essential for ERL1 internalization induced by EPF1 but not by EPFL6. The peptide antagonist, Stomagen, triggers retention of ERL1 in the endoplasmic reticulum, likely coupled with reduced endocytosis. In contrast, the dominant-negative ERL1 remained dysfunctional in ligand-induced subcellular trafficking. Our study elucidates that multiple related yet unique peptides specify cell fate by deploying the differential subcellular dynamics of a single receptor.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. Source R codes are provided.
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Funding
Howard Hughes Medical Institute (TORII)
- Keiko U Torii
Gordon and Betty Moore Foundation (GBMF3035)
- Keiko U Torii
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Qi et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The manifold actions of signaling peptides on subcellular dynamics of a receptor specify stomatal cell fate
eLife 9:e58097.
https://doi.org/10.7554/eLife.58097
