(a) and global phosphorylation (b) in protein extracts of long bones from WT, Hyp and Fgf23-/- mice. Post-immunoprecipation (Post-IP) indicates that despite similar levels of OPN (c), …
(a) and change in phosphorylation (b) for WT and Opn KO groups. * indicates significance at p<0.05 and error bars represent standard deviation.
(a) and change in phosphorylation (b) after removal of phosphate groups (dephosphorylation) for WT and Opn KO groups. * indicates significance at p<0.05 and error bars represent standard deviation.
* Indicates significance at p<0.05 and error bars represent standard deviation.
Fracture toughness of phosphoryled WT and Opn KO mice.
* Indicates significance at p<0.05 and error bars represent standard deviation.
Fracture toughness of dephosphoryled WT and Opn KO mice.
Energies are normalized to dissipation levels in EDTA for OPN deposited on mica and pulled with a pristine AFM tip (pH 7.4) and to dissipation levels in H2O for OPN deposited on HA and pulled with a …
Energy dissipation of native (phosphorylated) and dephosphorylated OPN film on mica in EDTA and calcium solution.
Energy dissipation of native (phosphorylated) OPN film on HA under various pH and ionic conditions.
In protein system (A), phosphorylation tends to increase inter- and intrafilament interactions, hence the interfilament distance is reduced. In protein system (B), phosphorylation tends to create …
By continuing the increase in phosphorylation of WT bone, fracture toughness improves exponentially. There is no significant relationship between global phosphorylation and fracture toughness in Opn …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. musculus) | C57BL/6NCrl | Charles River | RRID:IMSR_CRL:27 | |
Genetic reagent (M. musculus) | B6.Cg-PhexHyp/J | Jackson Laboratory | Cat#: 000528 RRID:IMSR_JAX:000528 | Animals maintained in Dr M Mckee lab. |
Genetic reagent (M. musculus) | Fgf23-/- | PMID:15579309 | Animals were a gift from Dr. B. Lanske | |
Genetic reagent (M. musculus) | B6.129S6(Cg)-Spp1tm1Blh/J | PMID:9661074 | Animals were a gift from Dr S. Rittling. | |
Genetic Reagent (B. taurus) | Milk protein (Mammary gland) | PMID:8320368 | Provided by Dr ES Sorensen | |
Chemical compound, drug | Synthetic hydroxyapatite | Andriotis et al., 2010. Crystal Research and Technology | Produced by Dr N. Bouropoulos | |
Commercial assay or kit | pIMAGO-biotin HRP Detection | Tymora Analytical | Cat# 900–100 | |
Antibody | anti-OPN (goat polyclonal) | R and D Systems | Cat# AF808, RRID:AB_2194992 | (1:100,000 µL) |
Antibody | anti-phosphoserine (rabbit polyclonal) | Thermo Fisher Scientific | Cat# 61–8100, RRID:AB_2533940 | (1:2500 µL) |
Adhesive properties of native (phosphorylated) and dephosphorylated OPN film on mica.
Adhesive properties of native (phosphorylated) OPN film on HA.
Mean maximum force of native (phosphorylated) OPN film on HA.
Mean maximum force of native (phosphorylated) and dephosphorylated OPN film on mica.