Strain, strain background (Rattus norvegicus) | Sprague-Dawley | Charles River | CD-IGS | For primary hippocampal neuron cultures |
Strain, strain background (Mus musculus) | C57BL/6J | Jackson Laboratory | Cat# 00064 | For RNA for RT-PCR for cloning |
Cell line (Homo sapiens) | HEK293 | ATCC | Cat# CRL-1573 | |
Cell line (Cercopithecus aethiops) | COS7 | ATCC | Cat# CRL-1651 | |
Biological sample (Rattus norvegicus) | Primary hippocampal neurons | This paper | | Freshly isolated from Rattus norvegicus |
Antibody | anti-Myc (rabbit polyclonal) | Sigma | Cat# C3956; RRID:AB_439680 | (ICC 1:40000) |
Antibody | anti-neurexin (rabbit polyclonal) | Millipore | Cat# ABN161; RRID:AB_10917110 | (ICC 1:1000) |
Antibody | anti-MAP2 (chicken polyclonal) | Abcam | Cat# ab5392; RRID:AB_2138153 | (ICC 1:80000) |
Antibody | anti-VGlut1 (mouse monoclonal) | NeuroMab | Cat# N28/9; RRID:AB_2187693 | (ICC 1:4000) |
Antibody | anti-synapsin I (mouse monoclonal) | Synaptic Systems | Cat# 106 011; RRID:AB_2619772 | (ICC 1:20000) |
Antibody | anti-tau (mouse monoclonal) | Millipore | Cat# MAB3420; RRID:AB_94855 | (ICC 1: 2000) |
Antibody | anti-HA (rat monoclonal) | Roche | Cat# 1186743100; RRID:AB_390919 | (WB 1: 2000) |
Recombinant DNA reagent | pNICE-HA-NLGN1 | Scheiffele et al., 2000 | | To subclone NLGN1 isoforms |
Recombinant DNA reagent | pCAGGS-Myc-NLGN1(-A+B)WT | This paper | | To express Myc-NLGN1 -A |
Recombinant DNA reagent | pCAGGS-Myc-NLGN1(+A1+B)WT | This paper | | To express Myc-NLGN1 +A1 |
Recombinant DNA reagent | pCAGGS-Myc-NLGN1(+A2+B)WT | This paper | | To express Myc-NLGN1 +A2 |
Recombinant DNA reagent | pCAGGS-Myc-NLGN1(+A1A2+B)WT | This paper | | To express Myc-NLGN1 +A1A2 |
Recombinant DNA reagent | pCAGGS-Myc-NLGN1(-A+B)P89L | This paper | | To express Myc-NLGN1 P89L -A |
Recombinant DNA reagent | pCAGGS-CFP-P2A-Myc-NLGN1(-A+B)WT | This paper | | To express Myc-NLGN1 -A |
Recombinant DNA reagent | pCAGGS-CFP-P2A-Myc-NLGN1(-A+B)P89L | This paper | | To express Myc-NLGN1 P89L -A |
Recombinant DNA reagent | pCAGGS-CFP-P2A-Myc-NLGN1(+A1+B)P89L | This paper | | To express Myc-NLGN1 P89L +A1 |
Recombinant DNA reagent | pcDNA4-HA-ecto-NLGN1(-A+B)-His | This paper | | To express HA-NLGN1-His -A ectodomain |
Recombinant DNA reagent | pcDNA4-HA-ecto-NLGN1(+A1+B)-His | This paper | | To express HA-NLGN1-His +A1 ectodomain |
Recombinant DNA reagent | pcDNA4-HA-ecto-NLGN1(+A2+B)-His | Zhang et al., 2018 | | To express HA-NLGN1-His +A2 ectodomain |
Recombinant DNA reagent | pcDNA4-HA-ecto-NLGN1(+A1A2+B)-His | This paper | | To express HA-NLGN1-His +A1A2 ectodomain |
Recombinant DNA reagent | pCAGGS-Myc-Amigo | This paper | | To express Myc-Amigo |
Recombinant DNA reagent | Amigo-CFP | Siddiqui et al., 2010 | | To subclone Myc-Amigo |
Recombinant DNA reagent | Myc-CD4 | This paper | | To express Myc-CD4 |
Recombinant DNA reagent | HA-CD4 | Takahashi et al., 2011 | | To subclone Myc-CD4 |
Recombinant DNA reagent | pLL3.7(hSyn)-Myc-NLGN1*(-A+B)WT | This paper | | To express Myc-NLGN1* -A |
Recombinant DNA reagent | pLL3.7(hSyn)-Myc-NLGN1*(+A1+B)WT | This paper | | To express Myc-NLGN1* +A1 |
Recombinant DNA reagent | pLL3.7(hSyn)-CFP-P2A-Myc-NLGN1*(-A+B)P89L | This paper | | To express Myc-NLGN1* -A P89L |
Recombinant DNA reagent | pLL3.7(hSyn)-CFP-P2A-Myc-NLGN1*(+A1+B)P89L | This paper | | To express Myc-NLGN1* +A1 P89L |
Recombinant DNA reagent | pFB-hSyn-DIO-YFP-P2A-HA-NLGN1*(+A2+B) | Zhang et al., 2018 | | To subclone NLGN1* isoforms |
Recombinant DNA reagent | pFB-hSyn-DIO-YFP-P2A-HA-NLGN1*(-A+B) | This paper | | To express Cre-dependent HA-NLGN1* -A |
Recombinant DNA reagent | pFB-hSyn-DIO-YFP-P2A-HA-NLGN1*(+A1+B) | This paper | | To express Cre-dependent HA-NLGN1* +A1 |
Recombinant DNA reagent | pCAG-Cre | Addgene | Addgene# 13775 | To express Cre |
Recombinant DNA reagent | pLL3.7U6-shNlgn1-hSyn-YFP | Zhang et al., 2018 | | To express shNlgn1 |
Recombinant DNA reagent | pLL3.7U6-shNlgn1-hSyn-CFP | This paper | | To express shNlgn1 |
Recombinant DNA reagent | pLL3.7(hSyn)-MCS | This paper | | To maintain uniform DNA amounts for transfections |
Recombinant DNA reagent | pECFP-N1 | Clontech | | To express CFP |
Recombinant DNA reagent | AP-NLGN1*(-A) | This paper | | To express AP-NLGN1* -A |
Recombinant DNA reagent | AP-NLGN1*(+A1) | This paper | | To express AP-NLGN1* +A1 |
Recombinant DNA reagent | shNlgn1-GFP | Chamma et al., 2016 | | To express shNlgn1 |
Recombinant DNA reagent | dsRed-Homer1C | Mondin et al., 2011 | | To express dsRed-Homer1c |
Recombinant DNA reagent | BirAER | Howarth et al., 2006 | | To express BirAER |
Chemical compound, drug | Bicuculline methiodide | Abcam | Cat# ab120109 | |
Chemical compound, drug | Tetrodotoxin (TTX) | Abcam | Cat# ab120054 | |
Other | Ni-NTA agarose beads | QIAGEN | Ca# 30210 | |
Other | Heparin agarose | GE Healthcare | Cat# 17-0406-01 | |
Software, algorithm | WinLTP | WinLTP | RRID:SCR_008590 | |
Software, algorithm | MiniAnalysis | Synaptosoft | RRID:SCR_002184 | |
Software, algorithm | Fiji 64-bit | https://doi.org/10.1038/nmeth.2019 | RRID:SCR_002285 | |
Software, algorithm | Pymol | Pymol | RRID:SCR_000305 | |
Software, algorithm | OLego v1.1.2 | Wu et al., 2013 | RRID:SCR_005811 | |
Software, algorithm | R | R Foundation | RRID:SCR_001905 | |
Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798; SCR_000306 | |
Software, algorithm | GraphPad InStat | GraphPad | RRID:SCR_000306 | |
Software, algorithm | GraphPad SigmaPlot | GraphPad | RRID:SCR_000306 | |