Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?
- Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, United States
- Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, United States
- Department of Obstetrics and Gynecology, Wayne State University School of Medicine, United States
- Department of Obstetrics and Gynecology, University of Michigan, United States
- Department of Epidemiology and Biostatistics, Michigan State University, United States
- Detroit Medical Center, United States
- Department of Obstetrics and Gynecology, Florida International University, United States
- Department of Computer Science, Wayne State University College of Engineering, United States
- Department of Physiology, Wayne State University School of Medicine, United States
- Department of Biochemistry, Microbiology and Immunology, Wayne State University School of Medicine, United States
Figures
Figure 1 with 1 supplement
Transcriptional map of the human placenta, including the decidua, in the three trimesters of pregnancy.
(A) Uniform Manifold Approximation Plot (UMAP), where dots represent single cells/nuclei and are colored by cell type (abbreviations used are: STB, Syncytiotrophoblast; EVT, Extravillous trophoblast;…
Figure 1—figure supplement 1
UMAP highlighting cells expressing syncytiotrophoblast genes CGA + CSH1 + GH2 that are more efficiently captured when isolating nuclei in snRNA-seq.
Uniform Manifold Approximation Plot (UMAP), where dots represent single cells/nuclei and are colored the combined gene expression of CGA + CSH1 + GH2 and normalized to transcripts per million per …
Figure 2 with 2 supplements
Dot plot depicting the expression of different viral receptors/molecules used by SARS-CoV-2, CMV, and ZIKV.
Each row represents a different cell type, and columns are grouped first by virus type, receptor/molecule gene, and placental tissue/time-of sampling (1DP, 2DP and 3DP represent the first, second, …
Figure 2—figure supplement 1
Gene expression values for BSG across tissues collected by the GTEx project.
For each tissue, a violin plot and a boxplot describe the variation in gene expression levels for BSG across all of the samples collected by the project sorted left to right by median gene …
Figure 2—figure supplement 2
Dot plot depicting the expression of different viral receptors/molecules used by virus that caused congenital infection.
Each row represents a different cell type, and columns are grouped first by virus type, receptor/molecule gene, and placental tissue/time-of sampling (1DP, 2DP and 3DP represent the first, second …
Additional files
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Supplementary file 1
Summary of all the single cell resources analyzed using existing and new data.
- https://cdn.elifesciences.org/articles/58716/elife-58716-supp1-v3.xls
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Supplementary file 2
Clinical and demographic characteristics of the study population from which placental samples were collected for snRNAseq studies.
- https://cdn.elifesciences.org/articles/58716/elife-58716-supp2-v3.docx
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Supplementary file 3
Bulk gene expression data analysis of ACE2 and TMPRSS2 in the placental tissues.
- https://cdn.elifesciences.org/articles/58716/elife-58716-supp3-v3.docx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/58716/elife-58716-transrepform-v3.docx
