(a) Schematic of all-optical setup. Bottom left: Example of flexible ensemble photostimulation. Three 10 neuron groups in barrel cortex (red, green, blue circles joined by group centroids) were …
(a) Detection of indicator/opsin expression overlap. Suite2P followed by manual curation was used to detect functional GCaMP-expressing neurons from time-series data (top row) and Cellpose followed …
(a) Left: A cross-shaped grid of photostimulation targets was centred on a single target neuron and each photostimulation target was activated 10 times in random order (10 µm inter-target distance, …
(a) Typical training protocol to transition a naïve mouse to being able to detect two-photon stimulation via a series of one-photon priming phases. Note that exact duration of each component varied …
(a) Kv21-C1V1-mScarlet expression from an example four-plane imaging volume with stimulation targets overlaid in pink. Neurons are chosen arbitrarily, with the only criteria being that neurons are …
(a – b) Response rates for different 1P and 2P stimulus types from the first session using 2P stimuli, quantified as proportion of trials where animal licked (a) and d-prime relative to catch trials …
(a) Animals detect stimulation of somatic and non-somatic C1V1 to similar extents. (b) Animals respond slightly quicker to stimulation of non-somatic C1V1. (c) Reaction times are similarly …
(a) Example imaging volumes from an experiment showing 200 (top), 50 (middle) and 5 (bottom) targeted C1V1-expressing neurons. (b) Example lick raster concatenating an animal’s two psychometric …
(a) Example target zones. Left: section of a plane in a volumetric FOV showing Suite2P ROIs (coloured regions), Suite2P centroids (black dots), target co-ordinates (pink dots) and lateral extent of …
(a–b) Relationship between mean (a) and standard deviation (b) of reaction time and the number of activated target neurons.
(a) FOV from training sessions across 3 consecutive days where we stimulated the same 30 neurons each day. (b) Average behavioural response rates for 100 neuron, 30 neuron and catch trial conditions …
(a) The proportion of neurons activated across all neurons (targets and background) increases as more target neurons are activated. Inset right: average activation across all trial types is …
(a) The proportion of background neurons activated when different numbers of target neurons are activated on hits/false alarms (green) and misses/correct rejects (grey) on stimulus trials (error …
(a) Distribution of trial-wise responses on 50+ neuron stimulation trials calculated from raw (Raw; black) and neuropil subtracted (Sub; red) traces (N = 30,894 neurons, 1312 trials). A large …
(a) Lateral and axial spatial profile of activation (left) and suppression (right) relative to nearest target site co-ordinate: for each neuron plot its distance to nearest target site co-ordinate …
(a–c) Psychometric curve fits relating behavioural detection to the number of targets activated (a), the proportion of the background network activated (b) and the proportion of the background …