(A) COL1A1 and BGLAP immunohistochemical staining of the femur head from hip dysplasia and osteoporosis patients. The red arrow shows typical staining. Scale bar, (upper, 500 µm; lower, 50 µm). (B) …
Relative mRNA expression of GAS5, BGLAP, ALPL, and RUNX2 along with the day of human BMSCs osteogenic differentiation.
(A) The correlation of GAS5 and ALP assay during osteogenic differentiation of MSCs (n = 15). (B, C, D) The correlation of GAS5 and ALPL, BGLAP, and RUNX2 during osteogenic differentiation of MSCs …
(A) GAS5 siRNA knockdown efficiency tested by qRT-PCR. (B) ARS staining and quantification in the GAS5 knockdown or control group. Scale bar, 500 µm. (C) ALP staining and ALP tests in the GAS5 …
(A) Western blot analysis and quantification of osteogenesis markers (COL1A1, RUNX2, BGLAP) in GAS5 knockdown or overexpression conditions. (B) Quantification of Western blot of osteogenesis markers …
The quantification of BGLAP immunohistochemical staining of in vivo bone formation tests in GAS5 knockdown or overexpression treatment.
(A) Relative GAS5 expression in the overexpression and control groups. (B) ARS staining and quantification in the GAS5 overexpression or control group. Scale bar, 50 µm. (C) ALP staining and ALP …
(A) Western blot analysis and quantification of osteogenesis markers (COL1A1, RUNX2, BGLAP) in GAS5 knockdown or overexpression conditions. (B) Quantification of western blot of osteogenesis markers …
The quantification of BGLAP immunohistochemical staining of in vivo bone formation tests in GAS5 knockdown or overexpression treatment.
(A) Coomassie brilliant blue staining of RNA pull-down proteins in the GAS5 sense or antisense group. (B) The interaction between GAS5 and UPF1 was confirmed by Western blotting. (C, D) RIP assay …
ZBTB37 (zinc finger and BTB domain containing 37), RC3H1 (ring finger and CCCH-type domains 1), SERPINC1 (serpin family C member 1), ZBTB37 (zinc finger and BTB domain containing 37), TNN (tenascin …
The model was predicted on the RNAfold web server website (http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi). The rainbow color means the base-pair probabilities of the predicted folded …
(A) Western blot analysis of the activation pathway (pSMAD1/5/8, SMAD1) in GAS5 knockdown or overexpression conditions. (B) SMAD7 was regulated by GAS5 knockdown or overexpression by western blot. (C…
(A, B) Western blot analysis and quantification of the pathway (catenin, ERK, SMAD2/3, SMURF1, SMURF2) in GAS5 knockdown or overexpression conditions. (C) Quantification for western blot analysis of …
(A, B, C, D, E, F) Western blot analysis and quantification of BMP2, BMP4, BMP7, and SMAD6 in GAS5 knockdown or overexpression conditions.
(A) Representative micro-CT analysis of the three-dimensional reconstruction graph for Gas5+/- (n = 6) and WT mice (n = 6). (B) Representative H and E staining (upper) and Bglap immunohistochemical …
(A, B) Relative Gas5 expression in the tails of Gas5+/+ (n = 6) and Gas5+/- (n = 6) mice analyzed by qRT-PCR and agarose gel electrophoresis. (C) Relative mRNA expression of Gas5, Upf1 and Smad7 in …
The quantification of Bglap immunohistochemical staining of Gas5+/-mice and WT mice.
Western blot analysis of the pathway (β-catenin, ERK) in the protein from the tibia of Gas5+/+ (n = 6) and Gas5+/- mice (n = 6).
(A) Micro-CT analysis of the control mice (n = 6), osteoporosis mouse model (n = 6) or Gas5-overexpressing adenovirus-treated mice (n = 6). (B) H and E staining and Bglap immunohistochemical …
The quantification of Bglap immunohistochemical staining of the control mice (n = 6), osteoporosis mouse model (n = 6) or Gas5-overexpressing adenovirus-treated mice.
Primers of the analyzed genes.
The siRNA sequences of the analyzed genes.
Characteristics of the study subjects.
Characteristics of the 15 healthy donors from Center for Biotherapy, Sun Yat-sen Memorial Hospital.
Data of mass spectrometry.