1. Developmental Biology
  2. Immunology and Inflammation
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Developmental Immunology: An atlas for hemocytes in an insect

  1. Samuel Liegeois
  2. Dominique Ferrandon  Is a corresponding author
  1. Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, UPR 9022 du CNRS, France
  2. Sino-French Hoffmann Institute, Guangzhou Medical University, China
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Cite this article as: eLife 2020;9:e59113 doi: 10.7554/eLife.59113
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The complexity within hemocyte populations in D. melanogaster larvae revealed by single-cell RNA sequencing.

RNA-seq analysis was performed on cells taken from D. melanogaster larvae that were either: (i) healthy; (ii) had been wounded with a tungsten needle; (iii) had been infested with parasitoid wasps (left). The three types of larval hemocytes – plasmatocytes (PM), crystal cells (CC) and lamellocytes (LL) – had their RNA extracted and analyzed to distinguish subpopulations within these groups (center). 12 plasmatocyte subpopulations (PM1–12), two crystal cell subpopulations (CC1, CC2), and two lamellocyte subpopulations (LM1, LM2) were defined, based on the clusters they formed when their gene expression was analyzed (right). Gene expression in these subpopulations can be accessed at www.flyrnai.org/scRNA/blood.

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