The upper part of the figure shows the five biological layers. From each of the four omics layers (epigenetic, transcriptomic, proteomic, and metabolomic data), biological age was estimated, and …
The heatmap represents Spearman rank correlations between the five biological aging indicators, all corrected for sex. Out of 10 pairs, three are significant: transcriptomic vs epigenetic aging, …
For each of the associations between biological aging indicators and health determinants, the standardized beta and standard deviation derived from linear models were plotted. The significant …
Telomere Length | Epigenetic Aging | Transcriptomic Aging | Proteomic Aging | Metabolomic Aging | Composite Index | ||
---|---|---|---|---|---|---|---|
# Subjects | 2936 | 1130 | 1990 | 1837 | 2910 | 653 | |
Demographic | Sex (%female) | 66.00 | 65.00 | 67.00 | 67.00 | 66.00 | 66.00 |
Education years (mean) | 12.15 | 11.93 | 12.07 | 12.07 | 12.15 | 11.71 | |
Age (mean) | 41.81 | 41.53 | 38.71 | 41.37 | 41.94 | 41.23 | |
Lifestyle | Alcohol use (units per week, mean) | 6.24 | 6.54 | 6.38 | 6.39 | 6.29 | 6.48 |
Smoking (pack years, mean) | 11.00 | 11.43 | 11.84 | 10.37 | 11.12 | 10.90 | |
Physical activity (MET minutes per week, mean) | 3679.72 | 3638.54 | 3729.20 | 3741.00 | 3668.13 | 3525.05 | |
Somatic Health | BMI (mean) | 25.60 | 25.67 | 25.68 | 25.66 | 25.60 | 25.82 |
Physical disability (score, mean) | 24.40 | 29.45 | 26.00 | 23.22 | 24.45 | 30.27 | |
Lung capacity (PEF in liter/minute, mean) | 477.74 | 479.75 | 478.42 | 477.19 | 477.23 | 475.23 | |
Hand grip strength (kg, mean) | 37.06 | 37.77 | 37.08 | 37.46 | 37.05 | 37.74 | |
Cardiometabolic disease (%cases) | 18 | 18 | 18 | 18 | 18 | 17 | |
Respiratory disease (%cases) | 9 | 9 | 9 | 9 | 9 | 10 | |
Musculoskeletal disease (%cases) | 10 | 10 | 10 | 9 | 10 | 9 | |
Digestive disease (%cases) | 9 | 9 | 9 | 8 | 9 | 8 | |
Neurological disease (%cases) | 3 | 2 | 3 | 3 | 3 | 2 | |
Endocrine disease (%cases) | 3 | 3 | 3 | 3 | 3 | 4 | |
Cancer (%cases) | 7 | 8 | 7 | 7 | 7 | 8 | |
Metabolic syndrome (# components, mean) | 1.36 | 1.39 | 1.37 | 1.33 | 1.36 | 1.41 | |
# Chronic diseases (mean) | 0.61 | 0.62 | 0.62 | 0.58 | 0.61 | 0.63 | |
Mental Health | Current MDD (%cases) | 27 | 72 | 34 | 26 | 27 | 76 |
Depression severity (IDS, mean) | 21.46 | 25.80 | 22.91 | 20.96 | 21.48 | 26.67 | |
Childhood Trauma (score from 0-4, mean) | 0.91 | 0.97 | 1.00 | 0.87 | 0.92 | 1.01 |
For each biological aging indicator, linear models were fit with the health determinant as predictor, while controlling for sex. Beta’s and p-values from these models are presented here. In the 653 …
Telomere Length | N=2936 | Epigeneticclock | N=1130 | Transcriptomic Clock | N = 1990 | Proteomic Clock | N=1837 | Metabolomic Clock | N=2910 | Composite Index (sum) | N=653 | Composite Index (PC1) | N=653 | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Beta* | P | Beta | P | Beta | P | Beta | P | Beta | P | Beta | P | Beta | P | ||
Demographic | Sex (male/female) | -0.06 | 2.89E-04 | -0.10 | 4.65E-04 | -0.15 | 3.64E-11 | -0.03 | 1.46E-01 | -0.08 | 1.25E-05 | -0.18 | 2.33E-06 | -0.11 | 3.59E-03 |
Education (# years) | -0.03 | 1.12E-01 | -0.02 | 5.21E-01 | -0.01 | 6.37E-01 | -0.05 | 3.43E-02 | -0.03 | 8.22E-02 | -0.04 | 3.11E-01 | -0.05 | 2.27E-01 | |
Lifestyle | Alcohol use (units per week) | 0.03 | 1.05E-01 | -0.05 | 1.40E-01 | 0.00 | 9.21E-01 | 0.07 | 2.89E-03 | 0.04 | 4.57E-02 | 0.07 | 6.05E-02 | 0.09 | 1.50E-02 |
Smoking (pack years) | 0.06 | 3.11E-03 | 0.02 | 6.22E-01 | 0.05 | 1.55E-02 | 0.10 | 1.33E-05 | 0.05 | 5.09E-03 | 0.10 | 1.15E-02 | 0.12 | 2.85E-03 | |
Physical activity | 0.02 | 2.75E-01 | -0.06 | 3.88E-02 | -0.04 | 6.42E-02 | 0.03 | 1.51E-01 | 0.01 | 5.18E-01 | -0.04 | 3.62E-01 | 0.01 | 7.38E-01 | |
Somatic Health | BMI | 0.04 | 1.80E-02 | 0.09 | 3.94E-03 | 0.14 | 6.02E-10 | 0.12 | 9.82E-08 | 0.23 | 2.07E-35 | 0.24 | 2.32E-10 | 0.22 | 2.18E-09 |
Physical disability | 0.03 | 9.11E-02 | 0.11 | 1.41E-04 | 0.04 | 8.61E-02 | 0.04 | 7.42E-02 | -0.01 | 4.24E-01 | 0.10 | 7.38E-03 | 0.03 | 4.01E-01 | |
Lung capacity | 0.02 | 4.19E-01 | 0.03 | 4.65E-01 | 0.04 | 2.13E-01 | -0.04 | 1.51E-01 | 0.03 | 2.37E-01 | 0.03 | 5.34E-01 | -0.02 | 6.57E-01 | |
Hand grip strength | -0.02 | 3.33E-01 | -0.06 | 1.71E-01 | 0.03 | 3.52E-01 | 0.01 | 7.30E-01 | 0.03 | 2.24E-01 | -0.03 | 6.14E-01 | 0.03 | 6.20E-01 | |
Cardiometabolic disease (no/yes) | 0.02 | 3.37E-01 | 0.04 | 1.56E-01 | 0.03 | 1.44E-01 | 0.03 | 1.35E-01 | 0.05 | 3.94E-03 | 0.10 | 1.37E-02 | 0.08 | 3.19E-02 | |
Respiratory disease (no/yes) | -0.02 | 2.12E-01 | -0.01 | 6.34E-01 | 0.02 | 2.85E-01 | 0.03 | 1.27E-01 | 0.01 | 4.67E-01 | -0.03 | 4.70E-01 | 0.01 | 7.17E-01 | |
Musculoskeletal disease (no/yes) | 0.00 | 8.11E-01 | -0.01 | 7.37E-01 | 0.04 | 1.04E-01 | 0.02 | 4.36E-01 | 0.02 | 2.23E-01 | 0.09 | 2.27E-02 | 0.11 | 4.96E-03 | |
Digestive disease (no/yes) | 0.03 | 5.77E-02 | -0.02 | 5.71E-01 | 0.06 | 9.76E-03 | 0.06 | 1.21E-02 | 0.02 | 2.81E-01 | 0.05 | 2.01E-01 | 0.04 | 2.86E-01 | |
Neurological disease (no/yes) | -0.02 | 2.58E-01 | 0.02 | 5.60E-01 | 0.01 | 5.44E-01 | 0.02 | 2.84E-01 | 0.02 | 1.93E-01 | -0.04 | 2.64E-01 | -0.02 | 5.09E-01 | |
Endocrine disease (no/yes) | -0.01 | 4.45E-01 | 0.01 | 8.13E-01 | -0.01 | 5.75E-01 | 0.06 | 1.03E-02 | 0.03 | 1.23E-01 | 0.06 | 1.18E-01 | 0.09 | 1.64E-02 | |
Cancer (no/yes) | 0.00 | 9.66E-01 | 0.02 | 5.65E-01 | 0.02 | 4.88E-01 | 0.03 | 1.81E-01 | 0.02 | 2.01E-01 | 0.08 | 3.22E-02 | 0.07 | 5.00E-02 | |
Metabolic syndrome (# components) | 0.06 | 6.35E-04 | 0.04 | 1.46E-01 | 0.13 | 9.98E-09 | 0.13 | 5.34E-09 | 0.21 | 4.53E-29 | 0.28 | 9.10E-13 | 0.26 | 6.41E-12 | |
# Chronic diseases | 0.00 | 7.99E-01 | 0.03 | 3.63E-01 | 0.05 | 3.20E-02 | 0.09 | 1.24E-04 | 0.03 | 1.39E-01 | 0.06 | 1.26E-01 | 0.07 | 8.43E-02 | |
Mental Health | Current MDD (no/yes) | 0.03 | 1.59E-01 | 0.09 | 1.99E-03 | 0.07 | 1.68E-02 | 0.08 | 7.62E-03 | -0.03 | 1.61E-01 | 0.11 | 6.05E-03 | -0.12 | 2.29E-01 |
Depression severity | 0.04 | 2.40E-02 | 0.12 | 8.67E-05 | 0.03 | 2.76E-01 | 0.07 | 5.99E-03 | -0.02 | 3.74E-01 | 0.13 | 7.61E-04 | 0.05 | 1.87E-01 | |
Childhood Trauma | 0.01 | 4.54E-01 | 0.12 | 7.99E-05 | 0.03 | 2.06E-01 | 0.04 | 8.96E-02 | 0.04 | 2.46E-02 | 0.09 | 1.96E-02 | 0.07 | 7.19E-02 |
* Beta for telomere length was multiplied by -1 to compare with other biological clocks.
Bold indicates FDR<5%.
Data used for Figure 3.
Data used for Figure 4.
Associations between biological aging and medication use.
Associations between biological aging (individual indicators and composite index) and health determinants in 653 overlapping samples.
For each biological aging indicator linear models were fit with the health determinant as predictor, while controlling for sex. The analysis was limited to the 653 samples with all five data layers available. Beta’s and p-values from these models are presented here. * Beta for telomere length was multiplied by −1 to compare with other biological aging indicators. All measures are coded such that higher values indicate advanced biological aging. Bold indicates FDR < 5%.
Longitudinal analysis of biological aging and mortality and chronic disease onset.