Messenger (m) RNA expression of (A) ICAM-1, (B) VCAM-1, and (C) E-selectin from HUVECs pretreated with either E2C6 or control IgG before TNF-α stimulation for 24 hr (*p<0.05, **p<0.01, ***p<0.001, n = 5 per group, ns = not significant). Lung mRNA 16 hr after LPS induced endotoxemia for markers of vascular inflammation (D) ICAM-1, (E) VCAM-1, (F) E-selectin. (*p<0.05, **p<0.01, ***p<0.001, n = 5 per group, ns = not significant,) (G) Representative lung immunostaining for granulocyte differentiation antigen (Gr)−1 (red) was performed 16 hr after cecal ligation puncture (CLP) or sham surgery in E2C6 (10 mg/kg) or IgG control antibody (IgG)-treated mice (nuclear staining with 4′,6-diamidino-2-phenylindole, (blue), autofluorescence is shown in green). (n = 5) Scale bar 50 μm. (H) Semiquantification of whole lung cross-sections by evaluating Gr-1+ cells per high power field (HPF) (HPF = 40 × magnification) (*p<0.05, **p<0.01, ***p<0.001, n = 5 per group).