(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in both voltage and calcium traces. Blue traces show average intensity of region of interest (ROI) used to record eyelid position (eyeblink responses). Insets show zoomed voltage traces during air puff stimulus. Gray bars show full duration of air puff stimulus. Top example shows voltage and calcium during control conditions and bottom example shows recordings following AIDA application from the same Purkinje neuron (PN). (b) Mean eye responses from control (top) and AIDA (bottom) recordings (black trace shows mean; blue trace shows SD). (c) Time for eyelid to close after stimulus onset under control (red) and AIDA (gray) conditions. There was no significant difference in eyelid closure time following AIDA application; control (120 ± 28 ms, mean ± SD, n = 64, 6 PNs); AIDA (122 ± 68 ms, mean ± SD, n = 74, 6 PNs), Kolmogorov–Smirnov, p=0.29, n = 138, 6 PNs. (d) Maximum change in dendritic voltage (excluding spikes) measured within a 200 ms window after stimulus onset under control and AIDA conditions. Stimulus-evoked voltage signals did not change following AIDA application; control (5.3 ± 2.6% ΔF/F, mean ± SD, n = 65, 6 PNs) and AIDA (6.3 ± 5.8% ΔF/F, mean ± SD, n = 90, 6 PNs), unpaired t-test, p=0.17, n = 155, 6 PNs. Probability distributions for DCS and DS events during pre-stimulus (e) and stimulus (f) time windows, under control and AIDA conditions (as in [c] and [d]). AIDA did not alter the probability of DCS and DS events during either pre-stimulus or stimulus time windows (pre-stimulus, p=1.0); (stimulus, p=1.0), Kolmogorov–Smirnov, n = 155, 6 PNs. Vertical bars in probability distributions show bootstrapped SD. (g) Relationship between the time of maximum change in dendritic voltage (depolarizing, red; or hyperpolarizing, blue) after stimulus onset and the time for eyelid closure, under control (open circles, n = 64, 6 PNs) and AIDA conditions (diamonds, n = 74, 6 PNs). Symbols show mean and bars show SD.