Dendritic coincidence detection in Purkinje neurons of awake mice
- Optical Neuroimaging Unit, Okinawa Institute of Science and Technology Graduate University (OIST), Japan
Figures
Figure 1 with 1 supplement
Electrical stimulation of parallel fiber (PF) input evokes graded dendritic voltage and calcium signals and dendritic spikes (DSs) in Purkinje neuron (PN) dendrites.
(a) A chronic cranial window with access port on the cerebellar vermis lobule V was used for imaging from the dendrites of single PNs and allowed access to the brain via micropipette. The dashed box …
Figure 1—figure supplement 1
Micropipette injection into cerebellar granule cell (GC) layer guided by two-photon microscopy.
(a) For an estimate of the spatial extent of drug delivery into the cerebellar vermis, 50 μM Fluorescein–5–isothiocyanate (FITC) dissolved in saline was delivered using the same injection parameters …
Figure 2 with 8 supplements
Sensory stimulation evokes graded depolarizing and hyperpolarizing signals and calcium signals in Purkinje neuron (PN) dendrites.
(a) 2P image of the spiny dendrites of two PNs (PN1 and PN2) labeled with voltage sensitive dye ANNINE-6plus (red) and genetically encoded calcium indicator GCaMP6f (green), resulting in double …
Figure 2—figure supplement 1
No difference in dendritic voltage signaling or time to eyelid closure between first and last recordings.
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in …
Figure 2—figure supplement 2
Dendritic voltage signals are time locked to sensory stimuli and not voluntary eye blinks.
(a) Single trial recordings of simultaneous dendritic voltage (red trace) and calcium (green trace) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in …
Figure 2—figure supplement 3
Example linescan showing evoked hyperpolarizing voltage signal during a sensory stimulus.
(a) Single linescan recordings of simultaneous dendritic voltage (red channel) and calcium (green channel). (b) Corresponding spatially averaged recordings of simultaneous dendritic voltage (red …
Figure 2—figure supplement 4
Sensory stimulus-evoked dendritic voltage signals and their relationship with eyelid closure time and climbing fiber (CF)-evoked dendritic calcium signals.
Single trial dendritic voltage recordings were sorted based on the dendritic voltage response during the stimulus into the following groups: depolarized (‘dep.’), hyperpolarized (‘hyp.’), …
Figure 2—figure supplement 5
Isoflurane induced anesthesia blocks stimulus-evoked dendritic voltage and calcium signaling.
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in …
Figure 2—figure supplement 6
During sensory stimulation the average maximum change in dendritic voltage in our sampling of lateral lobule V is positive and dendritic calcium signals are enhanced.
(a) Maximum change in dendritic voltage (excluding spikes) during sensory stimulation measured from all recordings in which evoked dendritic complex spike (DCS) signals occur, n = 365, 51 Purkinje …
Figure 2—figure supplement 7
AMPA receptor antagonist, CNQX, blocks stimulus-evoked dendritic voltage and calcium signaling.
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). Blue traces show average intensity of region of interest (ROI…
Figure 2—figure supplement 8
Na+ channel antagonist, Lidocaine, blocks stimulus-evoked dendritic voltage and calcium signaling and slows eyelid closure time.
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in …
Figure 3
Sensory stimulation evokes graded voltage-dependent dendritic calcium signals in the absence of dendritic complex spikes (DCSs) and dendritic spikes (DS).
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces). DCS and DS events are visible in both voltage and calcium traces. Blue traces show average …
Figure 4 with 1 supplement
Dendritic complex spikes (DCSs) and dendritic spikes (DSs) triggered by coincident climbing fiber (CF) and parallel fiber (PF) synaptic input evoke supralinear calcium signals during sensory stimulation.
(a) A simultaneous recording of dendritic calcium (green trace) and voltage (red trace) during air puff sensory stimulation, showing dendritic complex spike (DCS; filled triangle) and dendritic …
Figure 4—figure supplement 1
Types of dendritic voltage and calcium signals in Purkinje neurons (PNs) of awake mice, resolved with fast (2 kHz) simultaneous voltage and calcium imaging.
(a) Single trial recording examples of non-evoked (left) and evoked (right) dendritic voltage spike signals (red) and corresponding dendritic calcium signals (green) during high frequency dendritic …
Figure 5 with 1 supplement
Supralinear dendritic signaling in Purkinje neuron (PN) dendrites during sensory-evoked stimulation is voltage and mGluR1 dependent.
(a) Single trial examples of non-evoked dendritic complex spike (DCS) signals sorted based on the number of spikelets showing dendritic voltage (red traces) and calcium (green traces). (b) Averages …
Figure 5—figure supplement 1
mGluR1 antagonist, AIDA, has no effect on evoked eye blink closure time, stimulus-evoked dendritic voltage signaling, or the probability of dendritic complex spike (DCS) and dendritic spike (DS) events.
(a) Single trial recordings of simultaneous dendritic voltage (red traces) and calcium (green traces) during sensory stimulation (stim.). DCSs, DSs and corresponding calcium signals are visible in …
