(A) Diagram of interaction of EDA, EDAR, and EDARADD at the epithelial placode. (B) Schematic of EDA protein domains, including the transmembrane domain (TM), furin cleavage site, collagen domain, and TNF domain. Exon 4 of the EDA gene encodes the collagen domain. Relative positions of guide RNAs are shown. (C) PCR fragments from six edited pigs from one litter. Lane two shows position of predicted wild-type product. Pigs 1–5 were male; pig six was female.