(A) In the genome, the promoter upstream of frr (the gene encoding RRF) was replaced by the ligand-inducible araBAD promoter. A FLAG tag and residues YALAA were added to facilitate RRF detection and target it for degradation by ClpXP. (B) RRF depletion was monitored over time using antibodies against the FLAG epitope. CysRS serves as a loading control. The samples are: Ara (cultured in media with 0.2% arabinose), Glc-1 (harvested 10 min after the 1st change to media with 0.2% glucose), and three samples harvested 15, 30, and 60 min after the 2nd change to media with glucose. (C) Growth conditions for the samples used to make sequencing libraries. Note that the cultures were collected at earlier time points compared to (B).