Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris
- University of Washington, United States
Abstract
This study provides transcriptomic characterization of the cells of the crista ampullaris, sensory structures at the base of the semicircular canals that are critical for vestibular function. We performed single cell RNA-seq on ampullae microdissected from E16, E18, P3 and P7 mice. Cluster analysis identified the hair cells, support cells and glia of the crista as well as dark cells and other nonsensory epithelial cells of the ampulla, mesenchymal cells, vascular cells, macrophages and melanocytes. Cluster-specific expression of genes predicted their spatially restricted domains of gene expression in the crista and ampulla. Analysis of cellular proportions across developmental time showed dynamics in cellular composition. The new cell types revealed by single cell RNA-seq could be important for understanding crista function and the markers identified in this study will enable the examination of their dynamics during development and disease.
Data availability
All RNA-Seq data has been ave been deposited in NCBI's Gene Expression Omnibus (Edgar et al., 2002) and are accessible through GEO Series accession number# GSE168901.
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Single-cell Transcriptomic Analysis of the Mouse Crista AmpullarisNCBI Gene Expression Omnibus, GSE168901.
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Characterization of cochlear development at the single cell levelNCBI Gene Expression Omnibus, GSE137299.
Article and author information
Author details
Olivia Bermingham-McDonogh
Funding
National Institutes of Health (R01DC017126)
- Olivia Bermingham-McDonogh
National Institutes of Health (R21DC018094)
- Brent A Wilkerson
National Institutes of Health (F32DC016480)
- Brent A Wilkerson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal work was approved by the Institutional Animal Care and Use committee under protocol number 3123-01 and conforms to the recommendations of the NIH for animal care and use. Every effort was made to minimize any distress to the mice. Euthanasia of mice was in accordance with the AVMA guidelines.
Copyright
© 2021, Wilkerson et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris
eLife 10:e60108.
https://doi.org/10.7554/eLife.60108
