Antibody | Neurofilament heavy chain (chicken polyclonal) | Aves Lab | NFH | 1:750 |
Antibody | Anti-chicken Cy3 (donkey polyclonal) | Jackson Immunoresearch | 703-165-155 | 1:200 |
Antibody | Iba1 (rabbit polyclonal) | Wako Chemicals | 019–19741 | 1:500 |
Antibody | F4/80 (Rat IgG2b monoclonal) | Thermo Fisher Scientific | ma1-91124 | 1:500 - 1:1000 |
Antibody | CD68 (rabbit polyclonal) | Abcam | ab125212 | 1:500 |
Antibody | GFAP (rabbit polyclonal) | DAKO | Z033429-2 | 1:500 |
Antibody | SCG10 (rabbit polyclonal) | Novus Biologicals | NBP149461 | 1:500 - 1:1000 |
Antibody | CTB (goat polyclonal) | List Biological Laboratories | 703 | 1:10,000 |
Antibody | CD11b (rabbit monoclonal) | Abcam | ab133357 | 1:200-1:1000 |
Antibody | ERK1/2 (rabbit polyclonal) | Cell Signaling | 9102 | 1:5000 |
Antibody | Anti-rabbit HRP (donkey polyclonal) | EMD Millipore | AP182P | 1:2000-1:10000 |
Antibody | CD16/32 (Rat-IgG2a monoclonal) | BD Pharmingen | 553141 | 1 µg / 1 million cells / 25 µL |
Antibody | CD11b-PE-Cy7 (Rat-IgG2b monoclonal) | Thermo Fisher Scientific | 25-0112-82 | 1:200 |
Antibody | Isotype Control-PE-Cy7 (Rat-IgG2b monoclonal) | Thermo Fisher Scientific | 25-4031-82 | 1:100 |
Antibody | CD45-e450 (Rat-IgG2b monoclonal) | Thermo Fisher Scientific | 48-0451-82 | 1:100 |
Antibody | Isotype Control-e450 (Rat-IgG2b monoclonal) | Thermo Fisher Scientific | 48-4031-82 | 1:100 |
Antibody | CD45.1-e450 (Mouse-IgG2a monoclonal) | Biolegend | 110721 | 1:100 |
Antibody | Isotype Control-e450 (Mouse-IgG2a monoclonal) | Biolegend | 400235 | 1:100 |
Antibody | CD45.2-APC (Mouse-IgG2a monoclonal) | Biolegend | 109813 | 1:100 |
Antibody | Isotype Control-APC (Mouse-IgG2a monoclonal) | Biolegend | 400221 | 1:100 |
Antibody | Ly6G-APC-Cy7 (Rat-IgG2a monoclonal) | BD Biosciences | 560600 | 1:100 |
Antibody | Isotype Control-APC-Cy7 (Rat-IgG2a monoclonal) | BD Biosciences | 552770 | 1:100 |
Antibody | CD11c-PerCP-Cy5.5 (ArmHam-IgG monoclonal) | Thermo Fisher Scientific | 45-0114-82 | 1:100 |
Antibody | Isotype Control-PerCP-Cy5.5 (ArmHam-IgG monoclonal) | Thermo Fisher Scientific | 45-4888-80 | 1:100 |
Antibody | Ly6C-FITC (Rat-IgM monoclonal) | BD Biosciences | 561085 | 1:100 |
Antibody | Isotype Control-FITC (Rat-IgM monoclonal) | BD Biosciences | 553942 | 1:100 |
Antibody | Iba1 (goat polyclonal) | Novus Biologicals | NB100-1028 | 1:200 |
Antibody | Anti-goat Alexa Fluor 488 (donkey polyclonal) | Jackson Immunoresearch | 705-545-147 | 1:200 |
Chemical Compound | TOPRO pan-nuclear stain | Thermo Fisher Scientific | T3605 | |
Chemical Compound | Fixable Viability Dye | Thermo Fisher Scientific | 65086614 | 1:500 |
Chemical Compound | Proteinase K | New England Biolabs | P8107S | |
Chemical Compound | 10 mM dNTP mix | Promega | C1141 | |
Chemical Compound | 5X Green GoTaq Buffer | Promega | M791A | |
Chemical Compound | GoTaq DNA polymerase | Promega | M3005 | |
Chemical Compound | Buprenorphine | Par Pharmaceutical | NDC12496-0757-1 | |
Chemical Compound | Ketamine | Par Pharmaceutical | NDC42023-115-10 | |
Chemical Compound | Xylazine | Akorn | NDC59399-110-20 | |
Chemical Compound | Isoflurane | McKesson Corporation | 667940172 | |
Chemical Compound | Rhodamine-conjugated dextran MW 3,000 (Microruby) | Life Technologies | D-7162 | |
Chemical Compound | Cholera toxin B (CTB) | Life Technologies | C34775 | |
Chemical Compound | Puralube Eye ointment | Dechra | NDC-17033-211-38 | |
Chemical Compound | N2 | Gibco | 17502048 | |
Chemical Compound | N1 | Sigma | N6530 | |
Chemical Compound | Leibovitz-15 (L-15) | Gibco | 21083–027 | |
Chemical Compound | Penicillin/Streptomycin | Life Technologies | 15140–122 | |
Chemical Compound | DMEM Ham’s F-12 | Gibco | 10565–018 | |
Chemical Compound | Fetal Bovine Serum | Atlanta Biologicals | S11550 | |
Chemical Compound | Cytosine arabinoside | Sigma-Aldrich | C1768 | |
Chemical Compound | Collagenase type 2 | Worthington Biochemical | LS004176 | |
Chemical Compound | PBS without Calcium, Magnesium | Gibco | 10010023 | |
Chemical Compound | Poly-L-lysine MW 70,000–150,000 | Sigma-Aldrich | P4707 | |
Chemical Compound | laminin | Sigma-Aldrich | L2020 | |
Chemical Compound | Paraformaldehyde | Sigma-Aldrich | 158127–500G | |
Chemical Compound | Triton-X100 | Sigma-Aldrich | T8787 | |
Chemical Compound | Bovine Serum Albumin (BSA) heat shock fraction V | Fisher Scientific | BP1600 | |
Chemical Compound | Hoechst 33342 | Invitrogen | H3570 | |
Chemical Compound | Tissue-Tek O.C.T. Compound | Electron Microscopy Sciences | 62550–01 | |
Chemical Compound | β-Glycerophosphate | Sigma-Aldrich | G9422-100G | |
Chemical Compound | Sodium Orthovanadate (Na3VO4) | Sigma-Aldrich | S6508-10G | |
Chemical Compound | Protease inhibitor cocktail | Sigma-Aldrich | P8340-5ML | |
Chemical Compound | DC Protein Assay Kit | Bio-Rad | 5000111 | |
Chemical Compound | 2x Laemmli sample buffer | Bio-Rad | 1610737 | |
Chemical Compound | β-Mercaptoethanol | EMD Millipore | 6010 | |
Chemical Compound | Blotting-grade blocker | Bio-Rad | 1706404 | |
Chemical Compound | SuperSignal West Pico PLUS Chemiluminescent Substrate | Thermo Fisher Scientific | 34580 | |
Chemical Compound | SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Fisher Scientific | 34095 | |
Chemical Compound | WesternSure PREMIUM Chemi Substrate | Li-Cor Biosciences | 926–95000 | |
Chemical Compound | Fixable Viability Dye eF506 | Thermo Fisher Scientific | 65-0866-14 | |
Chemical Compound | TRIzol | Thermo Fisher Scientific | 15596026 | |
Chemical Compound | Dispase | Sigma-Aldrich | D4693 | |
Chemical Compound | Actinomycin D | Sigma-Aldrich | A1410 | |
Chemical Compound | Percoll | Sigma-Aldrich | P4937 | |
Chemical Compound | MACS buffer | Miltenyi | 130-091-376 | |
Chemical Compound | Hanks balanced salt solution | Gibco | 14025092 | |
Chemical Compound | Sucrose | Fisher Scientific | S5-500 | |
Other | Pasteur pipette | VWR | 14673–010 | |
Other | Flat Bottom Cell Culture Plates, 24-well | Corning | 3524 | |
Other | Superfrost Plus Microscope Slides | FisherScientific | 12-550-15 | |
Other | Zeiss Axio Observer Z1 | Zeiss | 491912-0049-000 | |
Other | Zeiss Axiocam 503 mono camera | Zeiss | 426559-0000-000 | |
Other | EC PlnN 10x objective | Zeiss | 420341-9911-000 | |
Other | Motorized tissue homogenizer | RPI | 299200 | |
Other | Fisher Scientific Sonic Dismembrator | Fisher Scientific | Model 500 | |
Other | Refrigerated Centrifuge | Eppendorf | 5424R | |
Other | Photospectrometer | Molecular Devices | SpectraMax M5e | |
Other | PVDF membrane | EMD Millipore | IPVH00010 | |
Other | LI-COR C-Digit | Li-Cor Biosciences | CDG-001313 | |
Other | 70 µm cell strainer | Corning | 352350 | |
Other | Ammonium-Chloride- Potassium (ACK) Lysing Buffer | Gibco | A1049201 | |
Other | Clinical Centrifuge | Beckman Coulter | Allegra 6R | |
Other | 40 µm filter | BD Falcon | 352340 | |
Other | Myelin Removal Beads | Miltenyi | 130-096-731 | |
Other | MidiMACS separator | Miltenyi | 130-042-302 | |
Other | LS Columns | Miltenyi | 130-042-401 | |
Other | Hemacytometer | Millipore Sigma | Z359629 | |
Other | Chromium Next GEM Chip G | 10X Genomics, Inc | NC1000127 | |
Other | 10X Genomic Chromium Controller | 10X Genomics, Inc | N/A | |
Other | NovaSeq Illumina 6000 | Illumina | N/A | |
Other | Cryostat | Leica Biosystems | CM3050S | |
Other | 70 µm Cell Strainer | Corning | 352350 | |
Other | Confocal Microscope | Nikon | C1 | |
Other | Confocal Microscope | Leica Biosystems | SP8 | |
Genetic Reagent (Mus musculus C57BL/6) | Csf2-/- | Jackson Laboratories | Stock No: 026812 | PMID:8171324 MGI: J:17978 |
Genetic Reagent (Mus musculus C57BL/6) | ROSA26-TdTom | Jackson Laboratories | Stock No: 007576 | PMID:17868096 MGI: J:124702 |
Genetic Reagent (Mus musculus C57BL/6) | CD45.1 | Jackson Laboratories | Stock No: 002014 | PMID:11698303 MGI: J:109863 PMID:11994430 MGI: J:109854 PMID:12004082 MGI: J:109853 |
Genetic Reagent (Mus musculus C57BL/6) | Wildtype, WT | Taconics | B6NTac | |
Genetic Reagent (Mus musculus C57BL/6) | Arg1-eYFP | Jackson Laboratories | Stock No: 015857 | PMID:17450126 MGI: J:122735 |
Oligonucleotide | Csf2 Forward | Integrated DNA Technologies | N/A | 5’-GTGAAACACAAGTTACCACCTATG-3’ |
Oligonucleotide | Csf2 Reverse | Integrated DNA Technologies | N/A | 5’-TTTGTCTTCCGCTGTCCAA-3’ |
Oligonucleotide | Neomycin Forward | Integrated DNA Technologies | N/A | 5’-CTTGGGTGGAGAGGCTATTC-3’ |
Oligonucleotide | Neomycin Reverse | Integrated DNA Technologies | N/A | 5’-AGGTGAGATGACAGGAGATC-3’ |
Software | WIS-Neuromath | Weizmann Institute of Science | Version 3.4.8 | PMID:23055261 |
Software | Image Studio Software | Li-Cor Biosciences | Version 5.2.5 | |
Software | NovaSeq control software | Illumina | Version 1.6 | |
Software | Real Time Analysis (RTA) software | Illumina | Version 3.4.4 | |
Software | Cellranger | 10X Genomics, Inc | Version 3.1.0 | |
Software | FACSDiva | BD Biosciences | Version 7 | |
Software | FlowJo | FlowJo LLC | Version 10.6.2 | |
Software | Seurat | Satija Lab - New York Genome Center | Version 3.1.2 | |
Software | R | r-project.org | Version 3.6.2 | |
Software | Slingshot | Bioconductor | Version 1.4.0 | |
Software | Ranger | Comprehensive R Archive Network | Version 0.12.1 | |
Software | Prism | GraphPad | Version 7 and 8 | |
Software | Imaris | Bitplane | | |
Software | Leica Application Suite (LAS X) | Leica | | |
Software | Zen Application Software | Zeiss | Pro 3.8 | |
Other | SomnoSuite | Kent Scientific | SS-01 | |
Other | Povidone-Iodine Prep Pad | PDI Healthcare | B40600 | |
Other | Alcohol Prep, Sterile, Md, 2 Ply | Covidien | 6818 | |
Other | Fine Forceps Dumont #55 Dumoxel | Roboz Surgical Instrument | RS-5063 | |
Other | 7 mm Reflex Wound Clips | Cell Point Scientific | 203–1000 | |
Other | Micro Friedman Rongeur | Roboz Surgical Instrument | RS-8306 | |
Other | McPherson-Vannas Micro Dissecting Spring scissors | Roboz Surgical Instrument | RS-5600 | |
Other | COATED VICRYL (polyglactin 910) Suture | Ethicon | J463G | |
Other | dumont #7 curved forceps | Fine Science Tools | 11271–30 | |
Other | Miltex halsted mosquito forceps | Integra LifeSciences | 724 | |
Other | Nanofil 10 µL syringe | World Precision Instruments | NANOFIL | |
Other | 36 g beveled nanofil needle | World Precision Instruments | NF36BV-2 | |
Other | Non-absorbable sutures | Ethicon | 640G | |
Other | Absorbable sutures | Ethicon | J463G | |