Proofreading through spatial gradients
- California Institute of Technology, United States
- University of Chicago, United States
Abstract
Key enzymatic processes use the nonequilibrium error correction mechanism called kinetic proofreading to enhance their specificity. The applicability of traditional proofreading schemes, however, is limited since they typically require dedicated structural features in the enzyme, such as a nucleotide hydrolysis site or multiple intermediate conformations. Here, we explore an alternative conceptual mechanism that achieves error correction by having substrate binding and subsequent product formation occur at distinct physical locations. The time taken by the enzyme-substrate complex to diffuse from one location to another is leveraged to discard wrong substrates. This mechanism does not have the typical structural requirements, making it easier to overlook in experiments. We discuss how the length scales of molecular gradients dictate proofreading performance, and quantify the limitations imposed by realistic diffusion and reaction rates. Our work broadens the applicability of kinetic proofreading and sets the stage for studying spatial gradients as a possible route to specificity.
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All scripts used to generate the data for making the plots are provided in supporting files.
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Author details
Funding
James S. McDonnell Foundation
- Kabir Husain
Simons Foundation
- Arvind Murugan
John Templeton Foundation
- Rob Phillips
National Institute of General Medical Sciences
- Rob Phillips
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Galstyan et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Proofreading through spatial gradients
eLife 9:e60415.
https://doi.org/10.7554/eLife.60415
