(A) 21-min CBF time profiles (mean ± standard deviation) that were retrieved in a full mid-brain slice at the ages of 3, 6, 9, and 12 months old shows for wild-type (WT) and transgenic Swedish Dutch Iowa (Tg-SwDI) mice. CO2 was administered between minutes 7 and 14. (B) Boxplot representations of baseline CBF (average of the last 2.3 min before the start of CO2 administration) and CVR (ratio of average of the last 2.3 min during CO2 to baseline CBF). Circles and squares represent individual mice. No significant differences were observed between the two genotypes, but there was a significant effect of age (Friedman test, p=0.005 for CBF in WT, p=0.037 for CBF in TG, p=0.040 for CVR in WT). From the post hoc analysis, only the drop in CBF in WT mice between 3 and 6 months old (p=0.008) reached the Bonferroni-corrected significance threshold (p=0.017).