(a), Representative image of a PC filled with the low-affinity Ca2+ dye Fluo-5F taken with whole-cell recording. The schematics on the left describe the number of CF stimuli (1, 3, 5, 7, and 9) at 400 Hz. b, Representative aligned traces of CS recordings and PC Ca2+ traces measured by Fluo-5F in response to the indicated numbers of 400 Hz CF stimuli (1, 3, 5, 7, and 9). The length between the two red asterisks represents the duration of CS. c–e, CS duration (c), spikelet numbers, (d) and amplitudes of the post-synaptic PC Ca2+ transient (e) in response to different number of spikes in the CF burst stimuli. n = 7 recordings of seven independent cells from three mice. One-way ANOVA followed by Bonferroni test: *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.