(A) Representative current traces induced by capsaicin or its analogs recorded at +80 mV (black) and −80 mV (red). (B) Concentration-response curves (n = 5–8 cells). (C) Representative single-channel traces in the presence of a saturating concentration of capsaicin or its analogs at +80 mV. Upward deflection represents the channel in the open state. (D) All-point histogram of single-channel events induced by respective agonists. n = 3–4 cells. (E) Comparison of the open probability. (F) Conductance, Po determined from single-channel and macroscopic recordings, and EC50 values at +80 mV in the presence of a saturating concentration of each agonist (Cap, 10 µM; Cap-1, 10 µM; Cap+1, 100 µM; n = 3–5 cells). *, p<0.05.