Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia
- Aix Marseille University, CNRS UMR7288, France
- Muséum National d'Histoire Naturelle, France
Abstract
Epithelial tissues acquire their integrity and function through the apico-basal polarization of their constituent cells. Proteins of the PAR and Crumbs complexes are pivotal to epithelial polarization, but the mechanistic understanding of polarization is challenging to reach, largely because numerous potential interactions between these proteins and others have been found, without a clear hierarchy in importance. We identify the regionalized and segregated organization of members of the PAR and Crumbs complexes at epithelial apical junctions by imaging endogenous proteins using STED microscopy on Caco-2 cells, and human and murine intestinal samples. Proteins organize in submicrometric clusters, with PAR3 overlapping with the tight junction (TJ) while PALS1-PATJ and aPKC-PAR6β form segregated clusters that are apical of the TJ and present in an alternated pattern related to actin organization. CRB3A is also apical of the TJ and partially overlaps with other polarity proteins. Of the numerous potential interactions identified between polarity proteins, only PALS1-PATJ and aPKC-PAR6β are spatially relevant in the junctional area of mature epithelial cells, simplifying our view of how polarity proteins could cooperate to drive and maintain cell polarity.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Pierre-François Lenne
Funding
Agence Nationale de la Recherche (ANR-10-INBS-04)
- Pierre Mangeol
- Dominique Massey-Harroche
- Fabrice Richard
- Pierre-François Lenne
- André Le Bivic
Agence Nationale de la Recherche (ANR-17-CE14-0032)
- Pierre Mangeol
- Dominique Massey-Harroche
- André Le Bivic
Instituts thematiques multi-organismes (Plan Cancer)
- Pierre Mangeol
Agence Nationale de la Recherche (ANR-11-LABX-0054)
- Pierre Mangeol
- Dominique Massey-Harroche
- Fabrice Richard
- Pierre-François Lenne
- André Le Bivic
Agence Nationale de la Recherche (ANR-11-IDEX-0001-02)
- Pierre Mangeol
- Dominique Massey-Harroche
- Fabrice Richard
- Pierre-François Lenne
- André Le Bivic
Ligue Contre le Cancer (Post doctoral grant)
- Pierre Mangeol
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Human subjects: Human biopsies were obtained with the agreement IPC-CNRS-AMU 154736/MB between the lab of the authors and the Paoli-Calmettes institute (Marseilles, France).
Copyright
© 2022, Mangeol et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia
eLife 11:e62087.
https://doi.org/10.7554/eLife.62087
