Primary visual cortex (V1) in the mouse projects to numerous brain areas, including several secondary visual areas, frontal cortex, and basal ganglia. While it has been demonstrated that optogenetic silencing of V1 strongly impairs visually-guided behavior, it is not known which downstream areas are required for visual behaviors. Here we trained mice to perform a contrast-increment change detection task, for which substantial stimulus information is present in V1. Optogenetic silencing of visual responses in secondary visual areas revealed that their activity is required for even this simple visual task. In vivo electrophysiology showed that, although inhibiting secondary visual areas could produce some feedback effects in V1, the principal effect was profound suppression at the location of the optogenetic light. The results show that pathways through secondary visual areas are necessary for even simple visual behaviors.
- Mark H Histed
- Mark H Histed
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: All procedures were conducted in accordance with the guidelines and regulations of the National Institutes of Health, according to an approved institutional animal care and use committee (IACUC) protocol (UNCB01) of the National Institute of Mental Health Intramural Program.
- Tatiana Pasternak, National Institute of Neurological Disorders and Stroke, United States
- Received: August 15, 2020
- Accepted: January 29, 2021
- Accepted Manuscript published: February 1, 2021 (version 1)
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