Proteomic and transcriptomic profiling reveal different aspects of aging in the kidney
- The Jackson Laboratory, United States
- Vividion Therapeutics, United States
- University of Zurich, Switzerland
- Harvard Medical School, United States
Abstract
Little is known about the molecular changes that take place in the kidney during the aging process. In order to better understand these changes, we measured mRNA and protein levels in genetically diverse mice at different ages. We observed distinctive change in mRNA and protein levels as a function of age. Changes in both mRNA and protein are associated with increased immune infiltration and decreases in mitochondrial function. Proteins show a greater extent of change and reveal changes in a wide array of biological processes including unique, organ-specific features of aging in kidney. Most importantly, we observed functionally important age-related changes in protein that occur in the absence of corresponding changes in mRNA. Our findings suggest that mRNA profiling alone provides an incomplete picture of molecular aging in the kidney and that examination of changes in proteins is essential to understand aging processes that are not transcriptionally regulated.
Data availability
Source data and analysis scripts have been deposited with FigShare (10.6084/m9.figshare. 12894146). In addition, the transcript and protein data are available in an online tool that supports genetic mapping analysis (https://churchilllab.jax.org/qtlviewer/JAC/DOKidney).The RNA-seq data have been deposited in NCBI's Gene Expression Omnibus, accession number GSE121330 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121330). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD023823.
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Transcriptomic profiling reveals distinct modes of aging in the kidneyNCBI Gene Expression Omnibus, GSE121330.
Article and author information
Author details
Isabela Gerdes Gyuricza
Funding
National Institutes of Health (AG038070)
- Ron Korstanje
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: The Jackson Laboratory's Institutional Animal Care and Use Committee approved all reported mouse studies.(AUS#06005).
Reviewing Editor
- Jing-Dong Jackie Han, Chinese Academy of Sciences, China
Publication history
- Received: September 1, 2020
- Accepted: March 6, 2021
- Accepted Manuscript published: March 9, 2021 (version 1)
- Version of Record published: May 4, 2021 (version 2)
- Version of Record updated: February 2, 2022 (version 3)
Copyright
© 2021, Takemon et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Proteomic and transcriptomic profiling reveal different aspects of aging in the kidney
eLife 10:e62585.
https://doi.org/10.7554/eLife.62585
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