(A) Growth curves of wt and CebpbΔuORF (ΔuORF) male mice on HFD (wt, n = 10; CebpbΔuORF, n = 8). (B) Volume of total fat mass as measured by abdominal CT analyses (males,19 weeks; ND, n = 5; HFD, n …
Raw data related to Figure 1A–G.
(A) Daily food intake per mouse on HFD, normalized to body weight as determined over 18 weeks (males, wt, n = 9 mice / 4 cages; ΔuORF n = 7 mice / 2 cages). (B) Efficiency of caloric utilization in …
Raw data and calculations related to Figure 1A–C.
Histological hematoxylin and eosin (H&E) staining of epididymal WAT from (A) males (19 weeks HFD) and (B) females (19 weeks HFD). Quantification of the fat cell area is shown at the right (males: …
Raw data related to Figure 2A, B.
(A) Relative mRNA expression levels of the macrophage marker CD68 measured in the visceral fat of CebpbΔuORF (ΔuORF) and wt male mice on either normal diet (ND) or HFD (19 weeks; ND, n = 5; HFD, n = …
Raw data related to Figure 3A, C.
(A) Relative mRNA expression levels of the macrophage marker CD68 measured in the visceral fat of CebpbΔuORF (ΔuORF) and wt female mice on either normal diet (ND) or HFD (19 weeks; wt: ND, n = 7; …
Raw data related to Figure 4A, C.
Histological sections of liver from (A) males and (B) females of wt or CebpbΔuORF mice (ΔuORF) (19 weeks). Sections were stained with hematoxylin (blue) and Sudan III (males) or Oil-Red-O (females) …
Raw data related to Figure 5C, D.
(A) Histological sections of cardiac muscle and (B) skeletal muscle of wt or CebpbΔuORF male mice (ΔuORF) (19 weeks). Sections were stained with hematoxylin (blue) and Sudan III for red color lipid …
Raw data related to Figure 5—figure supplement 1C, D.
Intraperitoneal glucose tolerance test (IPGTT) with the calculated area under the curve (AUC) of CebpbΔuORF (A) male and (B) female (ΔuORF) and wt mice injected i.p. with glucose (2 g/kg) after a 16 …
Raw data related to Figure 6A–D.
Relative mRNA expression levels of the adipogenic transcription factors C/EBPα, PPARγ and SREBBP1c and key enzyme FAS measured visceral WAT of CebpbΔuORF (A) male and (B) female (ΔuORF) mice and wt …
Raw data related to Figure 7A, B.
(A) Immunoblot of the adipogenic transcription factors C/EBPα, PPARγ and SREBP1 and the key enzyme FAS performed with visceral WAT extracts from CebpbΔuORF male mice (ΔuORF) and wt mice on either …
(A) Immunoblots of C/EBPβ and GAPDH loading control performed with visceral WAT extracts from wt or CebpbΔuORF males on either normal diet (ND) or HFD (19 weeks). (B) Quantification of the LAP/LIP …
Raw data related to Figure 8B, C, E and F.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. musculus) | C/EBPβΔuORF | https://doi.org.10.1101/gad.557910https://doi.org.10.15252/embr.201439837 | males, back-crossed for 6 generations and females, back-crossed for 12 generations into C57BL/6 J background | |
Antibody | Anti-C/EBPβ (E299) (rabbit monoclonal) | Abcam | Cat# ab32358, RRID:AB_726796 | (1:1000) |
Antibody | Anti-C/EBPα (D56F10) (rabbit monoclonal) | Cell Signaling | Cat# 8178, RRID:AB_11178517 | (1:1000) |
Antibody | Anti-PPARγ (C26H12) (rabbit monoclonal) | Cell Signaling | Cat# 2435, RRID:AB_2166051 | (1:1000) |
Antibody | Anti-FAS (C20G5) (rabbit monoclonal) | Cell Signaling | Cat# 3180, RRID:AB_2100796 | (1:1000) |
Antibody | Anti-GAPDH (14 C10) (rabbit monoclonal) | Cell Signaling | Cat# 2118, RRID:AB_561053 | (1:1000) |
Antibody | Anti-SREBP1 (2 A4) (mouse monoclonal) | NeoMarkers | Cat# MS-1207-PO | (1:1000) |
Antibody | Anti-CD68 (E307V) (rabbit monoclonal) | Cell Signaling | Cat# 97,778 | (1:200) |
Antibody | Anti-rabbit IgG, HRP-conjugated (donkey polyclonal) | GE Healthcare | Cat#: NA934, RRID:AB_772206 | (1:5000) |
Antibody | Anti-mouse IgG, HRP-conjugated (sheep polyclonal) | GE Healthcare | Cat#: NXA931, RRID:AB_772209 | (1:5000) |
Antibody | Anti-rabbit IgG, biotin-conjugated (goat polyclonal) | Vector Labs | Cat#: BA-1000 | (1:250) |
Sequence-based reagent | CD68 (F) | https://doi.org.10.7554/eLife.34985.001 | PCR primer | 5’-GCCCACCAC CACCAGTCACG –3’ |
Sequence-based reagent | CD68 (R) | https://doi.org.10.7554/eLife.34985.001 | PCR primer | 5’GTGGTCCAG GGTGAGGGCC A-3’ |
Sequence-based reagent | PPARγ (F) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-GCCCTTTGG TGACTTTATGG –3’ |
Sequence-based reagent | PPARγ (R) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-CAGCAGGTT GTCTTGGATGT 3’ |
Sequence-based reagent | C/EBPα (F) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-CAAGAACAG CAACGAGTACC G-3’ |
Sequence-based reagent | C/EBPα (R) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-GTCACTGGT CAACTCCAGCA C-3’ |
Sequence-based reagent | SREBP1c (F) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-AACGTCACT TCCAGCTAGAC –3’ |
Sequence-based reagent | SREBP1c (R) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-CCACTAAGG TGCCTACAGAG C-3’ |
Sequence-based reagent | FAS (F) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-ACACAGCAA GGTGCTGGAG-3’ |
Sequence-based reagent | FAS (R) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-GTCCAGGCT GTGGTGACTCT –3’ |
Sequence-based reagent | TNFα (F) | This paper | PCR primer | 5’-CCAGACCCT CACACTCA-3’ |
Sequence-based reagent | TNFα (R) | This paper | PCR primer | 5’-CACTTGGTG GTTTGCTACGA C-3’ |
Sequence-based reagent | MCP1 (F) | This paper | PCR primer | 5‘-GCTGGAGAG CTACAAGAGGA TCA-3’ |
Sequence-based reagent | MCP1 (R) | This paper | PCR primer | 5‘-ACAGACCTC TCTCTTGAGCT TGGT-3’ |
Sequence-based reagent | IL-1β (F) | This paper | PCR primer | 5‘-GAAATGCCA CCTTTTGACAG TG-3’ |
Sequence-based reagent | IL-1β (R) | This paper | PCR primer | 5‘-TGGATGCTC TCATCAGGACA G-3’ |
Sequence-based reagent | IL-6 (F) | This paper | PCR primer | 5’-CCGGAGAGG AGACTTCACAG –3’ |
Sequence-based reagent | IL-6 (R) | This paper | PCR primer | 5’-TTCTGCAAG TGCATCATCGT –3’ |
Sequence-based reagent | GAPDH (F) | This paper | PCR primer | 5’-ATTGTCAGC AATGCATCCTG –3’ |
Sequence-based reagent | GAPDH (R) | This paper | PCR primer | 5’-ATGGACTGT GGTCATGAGC C-3’ |
Sequence-based reagent | β-actin (F) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5’-AGAGGGAAA TCGTGCGTGA C-3' |
Sequence-based reagent | β-actin (R) | https://doi.org.10.15252/embr.201439837 | PCR primer | 5'-CAATAGTGA TGACCTGGCC GT-3’ |
Commercial assay or kit | Vectastain ABC HRP Kit | Vector Labs | Cat#: PK-4000 | |
Commercial assay or kit | Western Lightning Plus ECL Reagent | Perkin Emer | Cat#: NEL103001EA | |
Commercial assay or kit | ECL Prime Western Blotting Reagent | GE Healthcare | Cat#: RPN2236 | |
Commercial assay or kit | Restore Western Blot Stripping buffer | Thermo Fisher | Cat#: 21,063 | |
Commercial assay or kit | QIAzol Lysis re-agent | QIAGEN | Cat#: ID:79,306 | |
Commercial assay or kit | RNeasy Lipid Tissue Mini kit | QIAGEN | Cat#: ID:74,804 | |
Commercial assay or kit | Rneasy Plus Mini kit | QIAGEN | Cat#: ID:74,134 | |
Commercial assay or kit | Transcriptor First Strand cDNA Synthesis kit | Roche | Cat#: 4379012001 | |
Commercial assay or kit | Light Cycler 480 SYBR Green I Master Mix | Roche | Cat#: 0470751600 | |
Chemical compound, drug | Insulin (human) | Lilly | Cat#: HI-210 | |
Chemical compound, drug | Sudan III | Sigma-Aldrich | Cat#: S4136 | |
Chemical compound, drug | Oil-Red-O | Sigma-Aldrich | Cat#: O0625 | |
Software, algorithm | GraphPad Prism 9.0 | Graphpad Software, La Jolla, CA | RRID:SCR_002798 | |
Software, algorithm | Image Quant LAS 4000 Mini Imager Software | GE Healthcare | RRID:SCR_014246 | |
Software, algorithm | ImageJ | https://doi.org.10.1186/s12859-017-1934-z | RRID:SCR_003070 |