TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

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Abstract

TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease with impaired organ growth and increased tumor formation. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we characterize these centriolar protein assemblies (Cenpas) to uncover the mechanism of action of TRIM37. We find that an atypical de novo assembly pathway can generate Cenpas that act as microtubule organizing centers (MTOCs), including in Mulibrey patient cells. Correlative light electron microscopy reveals that Cenpas are centriole-related or electron-dense structures with stripes. TRIM37 regulates the stability and solubility of Centrobin, which accumulates in elongated entities resembling the striped electron dense structures upon TRIM37 depletion. Furthermore, Cenpas formation upon TRIM37 depletion requires PLK4, as well as two parallel pathways relying respectively on Centrobin and PLK1. Overall, our work uncovers how TRIM37 prevents Cenpas formation, which would otherwise threaten genome integrity, including in Mulibrey patients.

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All data generated or analysed during this study are included in the manuscript and supporting files.

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Fernando R Balestra

Genome Biology Department, CABIMER-University of Seville, Seville, Spain

For correspondence
fernando.balestra@cabimer.es

Competing interests
The authors declare that no competing interests exist.
Andrés Domínguez-Calvo

Genome Biology Department, CABIMER-University of Seville, Seville, Spain

Competing interests
The authors declare that no competing interests exist.
Benita Wolf

Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-5673-4239
Coralie Busso

Swiss Institute of Experimental Cancer Research, Swiss Federal Institute of Technology, Lausanne, Switzerland

Competing interests
The authors declare that no competing interests exist.
Alizée Buff

Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland

Competing interests
The authors declare that no competing interests exist.
Tessa Averink

Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland

Competing interests
The authors declare that no competing interests exist.
Marita Lipsanen-Nyman

Children's Hospital, University of Helsinki, Helsinki, Finland

Competing interests
The authors declare that no competing interests exist.
Pablo Huertas

Genome Biology Department, CABIMER-University of Seville, Seville, Spain

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-1756-4449
Rosa M Ríos

Cell Dynamics and Signalling, CABIMER-University of Seville, Seville, Spain

Competing interests
The authors declare that no competing interests exist.
Pierre Gönczy

Swiss Institute of Experimental Cancer Research, Swiss Federal Institute of Technology, Lausanne, Switzerland

For correspondence
pierre.gonczy@epfl.ch

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-6305-6883

Funding

Krebsforschung Schweiz (KFS-3388-02-2014)

Pierre Gönczy

Marie Curie Actions (PIEF-GA-2013-629414)

Fernando R Balestra

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Human subjects: Fibroblast cultures were established from skin biopsy samples with approval by the Institutional Review Board of the Helsinki University Central Hospital (183/13/03/03/2009). The patients signed an informed consent for the use of fibroblast cultures.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.