Fourteen Wlsfl/-;Gfap-Cre and fifteen Wlsfl/+;Gfap-Cre mice were tested. (A) Overview of the behavioral protocol for experiments on the rotating circular arena. A red 60° sector indicates the location of the shock zone. A black 60° sector indicates that the shock was turned off and entrances into this region were counted. The arena rotated at 1 rpm from day two onward. On day 6, the new arena was in a different location (indicated by the dark box). (B) Examples of active place avoidance behavior (shown by the mouse’s trajectory in the room-frame), documented across the rotating arena protocol. Arrowheads show the direction of 1 rpm rotation. A red sector indicates the location of the 60° active shock zone, and a black sector indicates the zone location when the shock was turned off. (C) Path length measured across the rotating arena protocol. (D) Initial active place avoidance learning. Left, number of entrances (errors). Center, time to first enter the shock zone. Right, maximum time the shock zone was avoided. By all three measures of place avoidance, Wlsfl/-;Gfap-Cre mice perform more poorly than the Wlsfl+-;Gfap-Cre littermates. (E) Conflict in active place avoidance learning. Left, center, and right are as described for (D). (F) Extinction of the conditioned place avoidance. Left, center, and right are as described for (D). The mice partially extinguished their previously learned avoidance. (G) Subsequent active place avoidance learning in a novel environment. Left, center, and right are as described for (D). Bars indicate the mean ± SEM; ∗ indicates p<0.05 for comparisons between genotypes.