(A) Lean and diet-induced obese (DIO) mice were treated daily with either PBS or T and V. Two groups of T and V-treated DIO mice were subjected to 12 hr fasting before tissue procurement. After 2 weeks, all mice were sacrificed, hypothalamus tissue dissected, and RNA levels of POMC, CART, NPY, AgRP, and MCH measured by real-time PCR (RT-PCR). (B) DIO mice were subjected to daily intraperitoneal (i.p.) injection of T and V, oral administration of MCH receptor one inhibitor (MCHR1-I), or a combined treatment of both, T and V + MCHR1-I for a course of 2 weeks. Body weight and food consumption was measured and expressed as a contingency plot displaying total weight, percentage of starting weight, and food consumption per mouse. (C) DIO rats were subjected to combined i.p. TDCA/valine injection and intracerebral administration of recombinant MCH and weight loss was monitored for 2 weeks. Results are representative of at least three independent experiments. Column plots display mean with standard deviation. Statistical significance was determined using one-way analysis of variance (ANOVA). Asterisks indicate p-values: *p < 0.05, **p < 0.01, and ***p < 0.001. Only significant values are shown (n = 5–7 animals/group).