Dose-dependent action of the RNA binding protein FOX-1 to relay X-chromosome number and determine C. elegans sex

This table includes a complete list of primers.

This table lists the gene targets, the target-specific sequence for each Cas9 guide RNA, the figure in which the results are presented, the genomic coordinates corresponding to each guide RNA, and the reference name of each guide. Two guides were used together to delete the fox-1 gene, and two guides were used together to replace sequences within intron VI of xol-1. Coordinates are based on Wbcel235/c11 version of the C. elegans genome.

This table presents information for CRISPR/Cas9 genome editing experiments involving HDR using single-stranded repair templates. The table lists the gene targets that were edited, the figure in which the results are presented, the DNA sequences of the repair template, with SNPs highlighted in red letters and deletion junctions highlighted in blue letters, the reference names of related guides, and the reference names of the oligonucleotide repair template. Two guide RNAs were used together in combination with the repair template to delete the endogenous fox-1 gene, resulting in the allele fox-1(y793). The double-stranded repair templates used for inducing mutations in the DNA encoding intron VI of endogenous xol-1 are available upon request but are too long to include in this table.

The table lists the gene targets, the figure or table in which the results are presented, the sequences of oligonucleotides used to screen for CRISPR/Cas9 induced mutations or to determine sequences of resulting mutations, the reference name of each oligonucleotide, and the function of each oligonucleotide. For asd-1, the oligos were used to verify the construction of strains built with a pre-existing asd-1 mutation, not for identifying new mutations made using Cas9. The oligonucleotides BF-2507 and BF-2508 were used to synthesize xol-1 repair templates rather than to identify Cas9-induced mutations in intron VI.