Hormone-sensitive lipase couples intergenerational sterol metabolism to reproductive success
- University of Graz, Austria
- Max Planck Institute of Molecular Cell Biology and Genetics, Germany
- Max Planck Institute for Biology of Ageing, Germany
Abstract
Triacylglycerol (TG) and steryl ester (SE) lipid storage is a universal strategy to maintain organismal energy and membrane homeostasis. Cycles of building and mobilizing storage fat are fundamental in (re)distributing lipid substrates between tissues or to progress ontogenetic transitions. In this study we show that Hormone-sensitive lipase (Hsl) specifically controls SE mobilization to initiate intergenerational sterol transfer in Drosophila melanogaster. Tissue-autonomous Hsl functions in the maternal fat body and germline coordinately prevent adult SE overstorage and maximize sterol allocation to embryos. While Hsl-deficiency is largely dispensable for normal development on sterol-rich diets, animals depend on adipocyte Hsl for optimal fecundity when dietary sterol becomes limiting. Notably, accumulation of SE but not of TG is a characteristic of Hsl-deficient cells across phyla including murine white adipocytes. In summary, we identified Hsl as an ancestral regulator of SE degradation, which improves intergenerational sterol transfer and reproductive success in flies.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Oskar Knittelfelder
Funding
Austrian Science Fund (P28882-B21)
- Gabriele Schoiswohl
Deutsche Forschungsgemeinschaft (FOR 2682)
- Andrej Shevchenko
Deutsche Forschungsgemeinschaft (TRR83 (TP17))
- Andrej Shevchenko
Austrian Science Fund (M 2706-B34)
- Ingrid Pörnbacher
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal protocols were approved by the Austrian Federal Ministry for Science, Research, and Economy (protocol number BMWFW-66.007/0026/-WF/V/3b/2017) and the ethics committee of the University of Graz, and were conducted in compliance with the council of Europe Convention (ETS 123).
Reviewing Editor
- Joel K Elmquist, University of Texas Southwestern Medical Center, United States
Publication history
- Received: September 28, 2020
- Accepted: February 3, 2021
- Accepted Manuscript published: February 4, 2021 (version 1)
- Version of Record published: February 12, 2021 (version 2)
Copyright
© 2021, Heier et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Hormone-sensitive lipase couples intergenerational sterol metabolism to reproductive success
eLife 10:e63252.
https://doi.org/10.7554/eLife.63252
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