(A) v-GAAP (orange bar) is encoded by many orthopox species, including most camelpox strains, many cowpox strains, and at least one vaccinia strain. In camelpox viruses, v-GAAP is encoded on the left end of the genome; in all other strains, v-GAAP is on the right end. In all strains, v-GAAP is flanked by CrmE and vaccinia-B22R. In vaccinia strains that do not encode v-GAAP, CrmE is also missing, suggesting recombination of the region between B22R and B25R, which is truncated in the Lister strain. (B) Alignment of target site duplications (blue) and flanking sequence in 21 v-GAAP-encoding poxvirus strains. CMLV, camelpox; CPXV, cowpox; MPXV, monkeypox; VACV, vaccinia. Asterisks denote gene truncations.